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![EV SEC Columns [NBP3-11763] - Results and EV separation. EV SEC column was filled with 2 ml of medium from HCT116 cells, concentrated 10 folds by Tangential Flow Filtration (TFF)-EV concentrator (Catalog # NBP3-11762). 24 fractions (500 μl each one) have been collected and analyzed by ELISA assay (CD81 marker) and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 8 - 12 (turnaround time approximately 15 min), whereas circulating proteins corresponded to the fractions 14 - 24.](http://images.novusbio.com/fullsize/nbp3-11763_ev-sec-columns-612025131132..png "EV SEC Columns [NBP3-11763] - Results and EV separation. EV SEC column was filled with 2 ml of medium from HCT116 cells, concentrated 10 folds by Tangential Flow Filtration (TFF)-EV concentrator (Catalog # NBP3-11762). 24 fractions (500 μl each one) have been collected and analyzed by ELISA assay (CD81 marker) and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 8 - 12 (turnaround time approximately 15 min), whereas circulating proteins corresponded to the fractions 14 - 24.")
![EV SEC Columns [NBP3-11763] - Collected fractions were analyzed by NTA (Zetaview, Particle Metrix), ELISA and by BCA assay for determining EV and total protein content. EVs are eluted in fraction 2.](http://images.novusbio.com/fullsize/nbp3-11763_ev-sec-columns-612025131233..png)