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![Western Blot: PRMT5 Antibody [NBP1-81701] - Western blot analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NBP1-81701-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PRMT5 Antibody [NBP1-81701] - Western blot analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PRMT5 Antibody [NBP1-81701] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81701-img0008.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP1-81701] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP1-81701-img0001.jpg)
![Western Blot: PRMT5 Antibody [NBP1-81701] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NBP1-81701-img0007.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0005.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0002.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse middle brain. PRMT5 antibody [N3C3] dilution: 1:500.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0004.jpg)
![Western Blot: PRMT5 Antibody [NBP2-19937] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-91020242024610.jpg)
![Immunocytochemistry/ Immunofluorescence: PRMT5 Antibody [NBP2-19937] - PRMT5 antibody [N3C3] detects PRMT5 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRMT5 stained by PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-91020242041233.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - PRMT5 antibody [N3C3] detects PRMT5 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.PRMT5 stained by PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-910202420104420.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0009.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - MCF7 xenograft, using PRMT5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0002.jpg)
![Western Blot: PRMT5 Antibody [NBP2-19935] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N1C1] (NBP2-19935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19935_rabbit-polyclonal-prmt5-antibody-91020242091465.jpg)
![PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments.IP samples: Raji whole cell extractA. 35 ug Raji whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [N1C1] (NBP2-19935)7.5 % SDS-PAGEThe immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] (NBP2-19935) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-19935_rabbit-polyclonal-prmt5-antibody-immunoprecipitation-261120251820238.jpg)
![Western Blot: PRMT5 Antibody [NB110-40571] - Detection of Human and Mouse PRMT5 on HeLa whole cell lysate using NB110-40571. PRMT5 was also immunoprecipitated by another rabbit anti-PRMT5 antibodies and NB110-40570.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB110-40571-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PRMT5 Antibody [NB110-40571] - Detection of Human and Mouse PRMT5 on HeLa whole cell lysate using NB110-40571. PRMT5 was also immunoprecipitated by another rabbit anti-PRMT5 antibodies and NB110-40570.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PRMT5 Antibody [NB110-40571] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PRMT5 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NB110-40571-img0005.jpg)
![Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Western-Blot-NBP3-15758-img0003.jpg "Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded mouse testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0002.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded rat testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0001.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-26220241535578.jpg "Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PRMT5 Antibody (2H4) [NBP3-26367] - Positive Western Blot detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate. All lanes: PRMT5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 72, 54, 68, 67 kDa Observed band size: 73 kDa](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-28220241152517.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-56041] - Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-56041-img0001.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-56041] - Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.")