| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1919SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38285-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03346-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03850-20ul | |||
![Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0006.jpg "Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1919-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0009.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0010.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import‐defectiveASchematic representation of wild‐type & mutant COA7.BCellular protein extracts were isolated from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The samples were analyzed by reducing SDS–PAGE & Western blot.CCellular fractions were prepared from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The fractions were analyzed by reducing SDS–PAGE & Western blot. T, total; C, cytosol; M, mitochondria.DHEK293 cells that transiently expressed wild‐type or mutant COA7HIS were solubilized, & the affinity purification of COA7HIS was performed. The samples were analyzed by reducing & non‐reducing SDS–PAGE & Western blot. Load: 3%. Eluate: 100%.EEqual amounts of wild‐type & mutant radiolabeled [35S]COA7 precursors were imported into mitochondria isolated from HEK293 cells. The samples were analyzed by reducing SDS–PAGE & autoradiography. The results of three biological replicates were analyzed, quantified, & normalized to wild‐type COA7 at 45 min. The data are expressed as a mean ± SEM (n = 3). IAA, iodoacetamide.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30885959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1919_rabbit-polyclonal-mitofilin-antibody-310202415532097.jpg)
![Western Blot: Mitofilin Antibody [NBP2-38286] - Analysis using Anti-IMMT antibody NBP2-38286 (A) shows similar pattern to independent antibody NBP2-38285 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38286-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Mitofilin Antibody [NBP2-38286] - Analysis using Anti-IMMT antibody NBP2-38286 (A) shows similar pattern to independent antibody NBP2-38285 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38286] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38286-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0009.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0010.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human stomach shows strong grtanular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Mitofilin Antibody [NBP2-38285] - Analysis using Anti-IMMT antibody NBP2-38285 (A) shows similar pattern to independent antibody NBP2-38286 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38285-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38285] - Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38285-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0005.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0006.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of Mouse brain, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03346-img0003.jpg "Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of Mouse brain, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of various cell lines, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03346-img0002.jpg)
![Immunohistochemistry: Mitofilin Antibody - BSA Free [NBP3-03346] - Immunohistochemistry analysis of Mitofilin in paraffin-embedded rat heart tissue using Mitofilin Rabbit pAb (A2751) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03346_rabbit-polyclonal-mitofilin-antibody-52202519302993.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody - BSA Free [NBP3-03346] - Confocal immunofluorescence analysis of U2OS cells using Mitofilin Rabbit pAb (A2751) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03346_rabbit-polyclonal-mitofilin-antibody-52202519302926.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/antibody/nbp3-23409_mouse-mitofilin-mab-m027-207202313321516.png "Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23409_mouse-mitofilin-mab-m027-1992023199280.jpg)
![Western Blot: Mitofilin Antibody (M027) [NBP3-23409] - Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (NBP3-23409) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23409_mouse-mitofilin-mab-m027-19920231820291.jpg)
![Western Blot: Mitofilin Antibody [NBP3-03850] - Analysis of extracts of various cell lines, using Mitofilin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Ba](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03850-img0001.jpg "Western Blot: Mitofilin Antibody [NBP3-03850] - Analysis of extracts of various cell lines, using Mitofilin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Ba")
![SDS-Page: Recombinant Human Mitofilin Protein [H00010989-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010989-Q01-1.jpg "SDS-Page: Recombinant Human Mitofilin Protein [H00010989-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation:Mitofilin AntibodyNBP3-30256] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-30256 (lot NBP3-30256-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-30256 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30256_rabbit-mitofilin-pab-301220241524147.jpg "Immunoprecipitation:Mitofilin AntibodyNBP3-30256] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-30256 (lot NBP3-30256-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-30256 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-29603]-Detection of human IMMT by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IMMT antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29603_rabbit-mitofilin-pab-231202512101653.jpg "Western Blot:[NBP3-29603]-Detection of human IMMT by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IMMT antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:Mitofilin AntibodyNBP3-29812] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-29812 (lot NBP3-29812-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-29812 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29812_rabbit-mitofilin-pab-30122024188590.jpg "Immunoprecipitation:Mitofilin AntibodyNBP3-29812] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-29812 (lot NBP3-29812-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-29812 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")