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![Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/BRCA1-Antibody-Western-Blot-NB100-199-img0004.jpg "Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds")
![Western Blot: BRCA1 Antibody [NB100-199] - Detection of Human BRCA1 by Western Blot and Immunoprecipitation. Sample: Nuclear extract from HeLa cells. Affinity purified rabbit anti-BRCA1 NB100-199 used at 1 mcg/ml for WB and 4 mcg for IP.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Western-Blot-NB100-199-img0003.jpg)
![Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Western-Blot-NB100-68208-img0004.jpg "Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Immunoprecipitation-NB100-68208-img0005.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NBP2-32073-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32073 used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), NBP2-32073 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NBP2-32073-img0002.jpg)
![Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32072. For blotting immunoprecipitated KAP-1, NBP2-32072 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32072-img0003.jpg)
![Western Blot: ZNRF2 Antibody [NBP1-28715] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZNRF2 antibody NBP1-28715 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. ZNRF2 was also immunoprecipitated by rabbit anti-ZNRF2 antibody NBP1-28697, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/ZNRF2-Antibody-Western-Blot-NBP1-28715-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZNRF2 Antibody [NBP1-28715] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZNRF2 antibody NBP1-28715 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. ZNRF2 was also immunoprecipitated by rabbit anti-ZNRF2 antibody NBP1-28697, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: RNF166 Antibody [NBP3-04262] - Analysis of extracts of various cell lines, using RNF166 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basi](http://images.novusbio.com/fullsize/RNF166-Antibody-Western-Blot-NBP3-04262-img0001.jpg "Western Blot: RNF166 Antibody [NBP3-04262] - Analysis of extracts of various cell lines, using RNF166 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basi")
![Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-2822024115250.jpg "Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa")
![Flow Cytometry: EGR2 Antibody (27C3) [NBP3-26605] - Overlay Peak curve showing SH-SY5Y cells stained with EGR2 Antibody (27C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-282202414312741.jpg)
![Immunoprecipitation: DAB2IP Antibody - BSA Free [NBP3-48050] - IP Result of anti-DAB2IP (IP:, 4ug; Detection: 1:1000) with mouse brain tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-48050_rabbit-dab2ip-pab-bsa-free-28420251154186.jpg "Immunoprecipitation: DAB2IP Antibody - BSA Free [NBP3-48050] - IP Result of anti-DAB2IP (IP:, 4ug; Detection: 1:1000) with mouse brain tissue lysate 4000ug.")
![Western Blot: DAB2IP Antibody - BSA Free [NBP3-48050] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (DAB2IP Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-48050_rabbit-dab2ip-pab-bsa-free-284202511463159.jpg)
![Western Blot: PCGF6 Antibody (SR2460) [NBP3-27743] - Western blot analysis of PC-12 lysates](http://images.novusbio.com/fullsize/nbp3-27743_rabbit-pcgf6-mab-sr2460-7520241013343.jpg "Western Blot: PCGF6 Antibody (SR2460) [NBP3-27743] - Western blot analysis of PC-12 lysates")
![Western Blot: VPS11 Antibody (SR1048) [NBP3-22131] - Western blot analysis using NBP3-22131 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22131_rabbit-vps11-mab-sr1048-5720231721518.jpg "Western Blot: VPS11 Antibody (SR1048) [NBP3-22131] - Western blot analysis using NBP3-22131 in K562 cell lysate.")
![Western Blot: TRAF-4 Antibody (SR1969) [NBP3-21648] - Western blot analysis using NBP3-21648 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21648_rabbit-traf-4-mab-sr1969-572023153124.jpg "Western Blot: TRAF-4 Antibody (SR1969) [NBP3-21648] - Western blot analysis using NBP3-21648 in HeLa cell lysate.")