| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08229 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20946 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58951-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58122-25ul | |||
![Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: MCF7 cell lysate Primary Ab: 3ug/ml NBP3-42133; Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-61220242151864.jpg "Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: MCF7 cell lysate Primary Ab: 3ug/ml NBP3-42133; Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Wnt-3a Antibody (C2) [NBP3-42133] - DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml NBP3-42133; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-612202421395343.jpg)
![Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: Recombinant Wnt-3a, Human.](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-61220242116236.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-288202412442968.jpg "Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)
![Western Blot: PPP3R2 Antibody - BSA Free [NBP3-46910] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (PPP3R2 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-46910_rabbit-ppp3r2-pab-bsa-free-284202512343883.jpg "Western Blot: PPP3R2 Antibody - BSA Free [NBP3-46910] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (PPP3R2 antibody) at dilution of 1:500")
![Immunohistochemistry: PPP3R2 Antibody - BSA Free [NBP3-46910] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (PPP3R2 Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-46910_rabbit-ppp3r2-pab-bsa-free-28420251235619.jpg)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-133-Immunohistochemistry-Paraffin-NBP3-07334-img0001.jpg "Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).")
![Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).](http://images.novusbio.com/fullsize/nbp3-07334_mouse-monoclonal-pkc-alpha-antibody-133-17720241044642.jpg)
![Western Blot: Phospholipase C beta 2 Antibody [NBP2-58951] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Western-Blot-NBP2-58951-img0002.jpg "Western Blot: Phospholipase C beta 2 Antibody [NBP2-58951] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Phospholipase C beta 2 Antibody [NBP2-58951] - Staining of human cell line PC-3 shows localization to cytosol.](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58951-img0001.jpg)
![Western Blot: FRAT1 Antibody [NBP2-58219] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/FRAT1-Antibody-Western-Blot-NBP2-58219-img0002.jpg "Western Blot: FRAT1 Antibody [NBP2-58219] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: FRAT1 Antibody [NBP2-58219] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/FRAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58219-img0001.jpg)
![Western Blot: Dishevelled-2 Antibody [NBP2-56345] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP2-56345-img0002.jpg "Western Blot: Dishevelled-2 Antibody [NBP2-56345] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Dishevelled-2 Antibody [NBP2-56345] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56345-img0001.jpg)
![Western Blot: ROCK2 Antibody [NBP2-58122] - Analysis using Anti-ROCK2 antibody NBP2-58122 (A) shows similar pattern to independent antibody NBP1-86124 (B).](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-58122-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ROCK2 Antibody [NBP2-58122] - Analysis using Anti-ROCK2 antibody NBP2-58122 (A) shows similar pattern to independent antibody NBP1-86124 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-58122] - Staining of human cell line HeLa shows localization to cytosol.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58122-img0001.jpg)