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![Western Blot: PPP2R5B Antibody [NBP1-31043] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (NBP1-31043). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PPP2R5B-Antibody-Western-Blot-NBP1-31043-img0003.jpg "Western Blot: PPP2R5B Antibody [NBP1-31043] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (NBP1-31043). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-4H8-Western-Blot-NBP3-15237-img0002.jpg "Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.")
![Flow Cytometry: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Overlay histogram showing HepG2 cells stained with Lgr5/GPR49 antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-4H8-Flow-Cytometry-NBP3-15237-img0001.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-20244-img0001.jpg "Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: RUVBL1 Antibody [NBP2-20244] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20244_rabbit-polyclonal-ruvbl1-antibody-1610202419405763.jpg)
![Western Blot: Skp1 Antibody [NBP2-20381] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SKP1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Skp1-Antibody-Western-Blot-NBP2-20381-img0002.jpg "Western Blot: Skp1 Antibody [NBP2-20381] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SKP1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CaM Kinase II alpha [p Thr 286] Antibody (SR1853) [NBP3-22608] - Western blot analysis using NBP3-22608 in Mouse brain treated with Lambda phosphatase cell lysate.](http://images.novusbio.com/fullsize/nbp3-22608_rabbit-cam-kinase-ii-alpha-p-thr-286-mab-sr1853-672023106231.jpg "Western Blot: CaM Kinase II alpha [p Thr 286] Antibody (SR1853) [NBP3-22608] - Western blot analysis using NBP3-22608 in Mouse brain treated with Lambda phosphatase cell lysate.")
![Western Blot: PRICKLE1 Antibody - BSA Free [NBP3-46898] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PRICKLE1 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-46898_rabbit-prickle1-pab-bsa-free-28420251221128.jpg "Western Blot: PRICKLE1 Antibody - BSA Free [NBP3-46898] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PRICKLE1 antibody) at dilution of 1:1000")
![Immunoprecipitation: PRICKLE1 Antibody - BSA Free [NBP3-46898] - IP Result of anti-PRICKLE1 (IP:, 3ug; Detection: 1:1000) with mouse brain tissue lysate 7000ug.](http://images.novusbio.com/fullsize/nbp3-46898_rabbit-prickle1-pab-bsa-free-28420251221183.jpg)
![Western Blot: Wnt-7b Antibody [H00007477-B01P] - Analysis of WNT7B expression in transfected 293T cell line by WNT7B polyclonal antibody. Lane 1: WNT7B transfected lysate(38.39 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Wnt-7b-Antibody-Western-Blot-H00007477-B01P-img0003.jpg "Western Blot: Wnt-7b Antibody [H00007477-B01P] - Analysis of WNT7B expression in transfected 293T cell line by WNT7B polyclonal antibody. Lane 1: WNT7B transfected lysate(38.39 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation:RUVBL1 AntibodyNBP3-29820] -Detection of human RUVBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUVBL1 antibody NBP3-29820 (lot NBP3-29820-1) used for IP at 6 µg per reaction. RUVBL1 was also immunoprecipitated by rabbit anti-RUVBL1.. For blotting immunoprecipitated RUVBL1, NBP3-29820 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29820_rabbit-ruvbl1-pab-301220242041573.jpg "Immunoprecipitation:RUVBL1 AntibodyNBP3-29820] -Detection of human RUVBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUVBL1 antibody NBP3-29820 (lot NBP3-29820-1) used for IP at 6 µg per reaction. RUVBL1 was also immunoprecipitated by rabbit anti-RUVBL1.. For blotting immunoprecipitated RUVBL1, NBP3-29820 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (NBP3-25756) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25756_rabbit-wnt-8b-mab-hl1549-azide-and-bsa-free-212024940825.jpg "Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (NBP3-25756) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (NBP3-25756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25756_rabbit-wnt-8b-mab-hl1549-azide-and-bsa-free-2120249415926.jpg)
![Western Blot: Cyclin D2 Antibody (5A6) [NBP2-46103] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D2.](http://images.novusbio.com/fullsize/Cyclin-D2-Antibody-5A6-Western-Blot-NBP2-46103-img0001.jpg "Western Blot: Cyclin D2 Antibody (5A6) [NBP2-46103] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D2.")
![Western Blot: FRAT2 Antibody [NB100-1009] - Analysis of FRAT2/GSK-3 in Human Heart lysate using NB100-1009 at 2 ug/ ml. Primary incubated for 1 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/FRAT2-Antibody-Western-Blot-NB100-1009-img0003.jpg "Western Blot: FRAT2 Antibody [NB100-1009] - Analysis of FRAT2/GSK-3 in Human Heart lysate using NB100-1009 at 2 ug/ ml. Primary incubated for 1 hour. Detected using chemiluminescence.")