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![Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/AKT1-p-Ser129-Antibody-7M4I8-Western-Blot-NBP3-16908-img0001.jpg "Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Met (c-Met) Overexpression Lysate (Adult Normal) [NBP2-11373] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Met (c-Met).](http://images.novusbio.com/fullsize/Met-c-Met-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11373-img0001.jpg "Western Blot: Met (c-Met) Overexpression Lysate (Adult Normal) [NBP2-11373] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Met (c-Met).")
![Western Blot: Src Overexpression Lysate (Adult Normal) [NBP2-07751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Src.](http://images.novusbio.com/fullsize/Src-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07751-img0001.jpg "Western Blot: Src Overexpression Lysate (Adult Normal) [NBP2-07751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Src.")
![Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/Insulin-Receptor-Beta-[p-Thr1160]-Antibody-Western-Blot-NBP2-60777-img0001.jpg "Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: Jak3 Antibody [NBP3-12702] - Lane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:125 kDaObserved band size:130 kDa](http://images.novusbio.com/fullsize/Jak3-Antibody-Western-Blot-NBP3-12702-img0001.jpg "Western Blot: Jak3 Antibody [NBP3-12702] - Lane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:125 kDaObserved band size:130 kDa")
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (001) [NBP2-89199] - Analysis of Human VEGFR2(CD309) expression on HUVEC cells. Cells were stained with purified anti-Human VEGFR2(CD309), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-001-Flow-Cytometry-NBP2-89199-img0001.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (001) [NBP2-89199] - Analysis of Human VEGFR2(CD309) expression on HUVEC cells. Cells were stained with purified anti-Human VEGFR2(CD309), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunoprecipitation: FAK Antibody [NBP3-30813] - Immunoprecipitation off AK using FAK antibodypreimmune serum (1) and affinity-purified antibody (2).](http://images.novusbio.com/fullsize/nbp3-30813_rabbit-fak-pab-811202420594221.jpg "Immunoprecipitation: FAK Antibody [NBP3-30813] - Immunoprecipitation off AK using FAK antibodypreimmune serum (1) and affinity-purified antibody (2).")
![Western Blot: EGFR [p Tyr845] Antibody (JE46-77) [NBP3-32761] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32761) at 1/1,000 dilution. Lane 1: A431 whole cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate Lane 3: A431 whole cell lysate treated with pp for 1 hour Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 134 kDa Observed band size: 180/75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32761) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32761_rabbit-egfr-p-tyr845-mab-je46-77-28820241244290.jpg "Western Blot: EGFR [p Tyr845] Antibody (JE46-77) [NBP3-32761] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32761) at 1/1,000 dilution. Lane 1: A431 whole cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate Lane 3: A431 whole cell lysate treated with pp for 1 hour Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 134 kDa Observed band size: 180/75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32761) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")