| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71331 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73713 | |||
![Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-12793-img0002.jpg "Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.")
![Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NBP2-12793-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0002.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0004.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70454-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0003.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunoprecipitation (IP) of CRYAB by using a monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70454-img0007.jpg)
![Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.](http://images.novusbio.com/fullsize/TCR-alpha-beta-Antibody-R73-Flow-Cytometry-NBP2-12941-img0002.jpg "Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.")
![Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat thymocytes with anti-rat TCR alpha/beta (R73) PE.](http://images.novusbio.com/fullsize/TCR-alpha-beta-Antibody-R73-Flow-Cytometry-NBP2-12941-img0001.jpg)
![Western Blot: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Western-Blot-NBP1-48040-img0022.jpg "Western Blot: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.")
![Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP1-48040-img0013.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human Ovary tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0021.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0014.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0015.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0016.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0017.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human breast tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0018.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human Kidney tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0019.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human lung tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0020.jpg)
![Immunoprecipitation: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Lane 1: Negative Control Lane 2: NBP1-48039 Lane 3: NBP1-48040 Lane 4: NBP1-48041 Lane 5: NBP1-48042 Lane 6: NBP1-48043 Lane 7: NBP1-48044 Lane 8: NBP1-48045](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunoprecipitation-NBP1-48040-img0023.jpg)
![Western Blot: YAP1 Antibody (10G4S10) [YAP1] - Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using [KO Validated] YAP1 Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517304998.jpg "<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody (10G4S10) [YAP1] - Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using [KO Validated] YAP1 Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517315554.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733585.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517304960.jpg)
![Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of HeLa cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251736120.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733574.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517352720.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517352748.jpg)
![Immunoprecipitation: YAP1 Antibody (10G4S10) [YAP1] - Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug YAP1 antibody . Western blot was performed from the immunoprecipitate using YAP1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517315557.jpg)
![Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of U20S cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733562.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [NBP3-15685] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-822025209898.jpg)
![Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Western-Blot-NBP3-11864-img0001.jpg "Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - ICC staining VEGFR1/Flt-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0003.jpg)
![Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Flow-Cytometry-NBP3-11864-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0005.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0002.jpg)
![SDS-Page: Recombinant Human YAP1 Protein [H00010413-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-YAP1-Protein-SDS-Page-H00010413-P01-img0002.jpg "SDS-Page: Recombinant Human YAP1 Protein [H00010413-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-simple-western-3032023112047.jpg "Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Flow-Cytometry-NBP2-76399-img0007.jpg)
![Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-NBP2-76399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-Paraffin-NBP2-76399-img0009.jpg)
![Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunoprecipitation-NBP2-76399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry-paraffin-732024101321..jpg)
![Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-western-blot-732024101522..jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0006.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0005.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-87000_rabbit-polyclonal-zap70-antibody-304202513131169.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.")
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] - FAK antibody detects FAK protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FAK stained by FAK antibody (NBP3-12897) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-12897_rabbit-fak-p-tyr-397-pab-910202419593568.jpg)
![Western Blot: PTP epsilon Antibody (5F2) [NBP2-02374] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilon.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Western-Blot-NBP2-02374-img0001.jpg "Western Blot: PTP epsilon Antibody (5F2) [NBP2-02374] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilon.")
![Immunocytochemistry/Immunofluorescence: PTP epsilon Antibody (5F2) [NBP2-02374] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP epsilon.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunocytochemistry-Immunofluorescence-NBP2-02374-img0008.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded prostate tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0007.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0002.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0003.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0004.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Kidney tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0005.jpg)
![Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded liver tissue using anti-PTP epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTP-epsilon-Antibody-5F2-Immunohistochemistry-Paraffin-NBP2-02374-img0006.jpg)