| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44913-0.02mg | |||
| Azide and BSA Free | NBP3-11559 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26303-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05396-20ul | |||
![Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Western-Blot-NBP1-77936-img0003.jpg "Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.")
![Immunohistochemistry-Paraffin: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Tissue: placental trophoblasts. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: EGFR pY1197 antibody at 5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: EGFR pY1197 is on the cell membrane. Staining: EGFR pY1197 as precipitated red signal with hematoxylin purple counterstain.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Immunohistochemistry-Paraffin-NBP1-77936-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913] - Formalin-fixed, paraffin-embedded human skin carcinoma stained with S100A9 Antibody (S100A9/1075).](http://images.novusbio.com/fullsize/S100A9-Antibody-S100A9-1075-Immunohistochemistry-Paraffin-NBP2-44913-img0002.jpg "Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913] - Formalin-fixed, paraffin-embedded human skin carcinoma stained with S100A9 Antibody (S100A9/1075).")
![Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with S100A9 Antibody (S100A9/1075).](http://images.novusbio.com/fullsize/S100A9-Antibody-S100A9-1075-Immunohistochemistry-Paraffin-NBP2-44913-img0001.jpg)
![Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate.<br /> All lanes: Src Antibody at 1.2ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 60, 61 KDa<br /> Observed band size: 60, 61 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202411472113.jpg "Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate.<br /> All lanes: Src Antibody at 1.2ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 60, 61 KDa<br /> Observed band size: 60, 61 KDa<br />")
![Immunocytochemistry/Immunofluorescence: Src Antibody (21H5) [NBP3-26303] - Staining of MCF-7 cells with Src Antibody (21H5) at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-28220241312247.jpg)
![Flow Cytometry: Src Antibody (21H5) [NBP3-26303] - Overlay histogram showing SH-SY5Y cells stained with Src Antibody (21H5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202414183653.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13229-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13229-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13229-img0003.jpg)
![Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13231] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Western-Blot-NBP3-13231-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13231] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1148] Antibody [NBP3-13231] - EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13231-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1148] Antibody [NBP3-13231] - EGFR (phospho Tyr1148) antibody detects EGFR protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunohistochemistry-Paraffin-NBP3-13231-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13267-img0002.jpg "Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunohistochemistry-Paraffin-NBP3-13267-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13266-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13266-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13266-img0003.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241252814.jpg "Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: PTP epsilon Antibody [NBP3-38398] - Western blot analysis of various lysates using PTP epsilon Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-1012202411531092.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241224188.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
![Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Src [p Tyr529] Antibody-Western Blot-NBP3-05396-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Biological Strategies Validation. </b>Western Blot: Src [p Tyr529] Antibody - Azide and BSA Free [Src] - Western blot analysis of lysates from A-431 cells, using Src Rabbit pAb at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-05396_rabbit-polyclonal-src-p-tyr529-antibody-52202516293989.jpg)