| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20097-0.025mg | |||
![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg)
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-2742023924237.png "Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-28B-8 (NBP3-20097) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202392428.png)
![Flow Cytometry: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28B-8 (NBP3-20097, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202396466.png)
![Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FHL1-Antibody-Western-Blot-NB100-1461-img0001.jpg "Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: FHL1 Antibody [NB100-1461] - Proteins interacting with HACE1 accumulate in Hace1-/-sTAC heart. Representative western blots show increased accumulation of some of the HACE1-interacting proteins in the Hace1-/-sTAC myocardium. n=3 mice per group, error bars represent s.e.m., P-value between KO and WT sTAC as indicated (one-way analysis of variance). GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms4430), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FHL1-Antibody-Western-Blot-NB100-1461-img0002.jpg)
![SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Beclin-1-Protein-SDS-Page-H00008678-P01-img0001.jpg "SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: RBBP6 Antibody (5A11) [H00005930-M01] - Analysis of monoclonal antibody to RBBP6 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RBBP6-Antibody-5A11-Immunocytochemistry-Immunofluorescence-H00005930-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: RBBP6 Antibody (5A11) [H00005930-M01] - Analysis of monoclonal antibody to RBBP6 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: RBBP6 Antibody (5A11) [H00005930-M01] - Analysis of monoclonal antibody to RBBP6 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RBBP6-Antibody-5A11-Immunohistochemistry-Paraffin-H00005930-M01-img0006.jpg)
![ELISA: RBBP6 Antibody (5A11) [H00005930-M01] - Detection limit for recombinant GST tagged RBBP6 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RBBP6-Antibody-5A11-ELISA-H00005930-M01-img0007.jpg)
![Western Blot: XRCC2 Antibody (2H4) [H00007516-M01] - Analysis of XRCC2 expression in transfected 293T cell line by XRCC2 monoclonal antibody (M01), clone 2H4.Lane 1: XRCC2 transfected lysate(32 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/XRCC2-Antibody-2H4-Western-Blot-H00007516-M01-img0005.jpg "Western Blot: XRCC2 Antibody (2H4) [H00007516-M01] - Analysis of XRCC2 expression in transfected 293T cell line by XRCC2 monoclonal antibody (M01), clone 2H4.Lane 1: XRCC2 transfected lysate(32 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: XRCC2 Antibody (2H4) [H00007516-M01] - Analysis of monoclonal antibody to XRCC2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/XRCC2-Antibody-2H4-Immunocytochemistry-Immunofluorescence-H00007516-M01-img0003.jpg)
![ELISA: XRCC2 Antibody (2H4) [H00007516-M01] - Detection limit for recombinant GST tagged XRCC2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/XRCC2-Antibody-2H4-ELISA-H00007516-M01-img0006.jpg)
![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - TOP3B monoclonal antibody (M01), clone 4F11 Analysis of TOP3B expression in K-562.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0007.jpg "Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - TOP3B monoclonal antibody (M01), clone 4F11 Analysis of TOP3B expression in K-562.")
![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0005.jpg)
![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.Lane 1: TOP3B transfected lysate(96.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0008.jpg)
![Sandwich ELISA: Topo III beta Antibody (4F11) [H00008940-M01] - Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Sandwich-ELISA-H00008940-M01-img0006.jpg)
![Western Blot: hHR23b Antibody (3H7) [H00005887-M10] - Analysis of RAD23B expression in transfected 293T cell line by RAD23B monoclonal antibody (M10), clone 3H7. Lane 1: RAD23B transfected lysatE (43.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/hHR23b-Antibody-3H7-Western-Blot-H00005887-M10-img0004.jpg "Western Blot: hHR23b Antibody (3H7) [H00005887-M10] - Analysis of RAD23B expression in transfected 293T cell line by RAD23B monoclonal antibody (M10), clone 3H7. Lane 1: RAD23B transfected lysatE (43.2 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: hHR23b Antibody (3H7) [H00005887-M10] - Analysis of monoclonal antibody to RAD23B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/hHR23b-Antibody-3H7-Immunohistochemistry-Paraffin-H00005887-M10-img0002.jpg)
![Sandwich ELISA: hHR23b Antibody (3H7) [H00005887-M10] - Detection limit for recombinant GST tagged RAD23B is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/hHR23b-Antibody-3H7-Sandwich-ELISA-H00005887-M10-img0003.jpg)