| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16955-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35254-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1312-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2748-0.025mg | |||
![Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p53-[p-Thr81]-Antibody-Western-Blot-NBP3-16955-img0001.jpg "Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179] - Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 Rabbit mAb (NBP3-16179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRAS40-Antibody-2Y8Y3-Western-Blot-NBP3-16179-img0001.jpg "Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179] - Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 Rabbit mAb (NBP3-16179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: NORE1 Antibody [NBP3-35254] - Western blot analysis of various lysates using NORE1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35254_rabbit-nore1-pab-1012202411392974.jpg "Western Blot: NORE1 Antibody [NBP3-35254] - Western blot analysis of various lysates using NORE1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: VEGF R1/Flt-1 Antibody (MAB0702) [NBP2-21963] - HUVECs, preincubated with anti-VEGFR1 at indicated dose for 20mins, were stimulated with human VEGF for 20 mins and then the level of p-VEGFR1 was detected using anti-phospho-VEGFR1 (Y1213).](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-MAB0702-Western-Blot-NBP2-21963-img0002.jpg "Western Blot: VEGF R1/Flt-1 Antibody (MAB0702) [NBP2-21963] - HUVECs, preincubated with anti-VEGFR1 at indicated dose for 20mins, were stimulated with human VEGF for 20 mins and then the level of p-VEGFR1 was detected using anti-phospho-VEGFR1 (Y1213).")
![Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32812_rabbit-tsc2-p-ser939-mab-je54-06-28820241244294.jpg "Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: FADD Antibody (JE32-76) [NBP3-32327] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32327) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (10 ug/Lane) Lane 2: C6 cell lysate (10 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32327) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32327_rabbit-fadd-mab-je32-76-278202415295569.jpg "Western Blot: FADD Antibody (JE32-76) [NBP3-32327] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32327) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (10 ug/Lane) Lane 2: C6 cell lysate (10 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32327) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NBP1-77978-img0001.jpg "Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.")
![Immunohistochemistry-Paraffin: TTC11 Antibody (D3) [NBP3-41932] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 20ug/ml NBP3-41932; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41932_mouse-ttc11-mab-d3-612202421331616.jpg "Immunohistochemistry-Paraffin: TTC11 Antibody (D3) [NBP3-41932] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 20ug/ml NBP3-41932; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")