| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02958-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93006-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98908-50ul | |||
![Western Blot: TATA binding protein TBP Antibody [NBP3-02958] - Analysis of extracts of various cell lines, using TATA binding protein TBP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP3-02958-img0003.jpg "Western Blot: TATA binding protein TBP Antibody [NBP3-02958] - Analysis of extracts of various cell lines, using TATA binding protein TBP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Western Blot: TATA binding protein TBP Antibody - BSA Free [NBP3-02958] - Western blot analysis of lysates from SK-OV-3 cells using TATA binding protein TBP Rabbit pAb(A2192) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (RM00020).Exposuretime:3s.](http://images.novusbio.com/fullsize/nbp3-02958_rabbit-polyclonal-tata-binding-protein-tbp-antibody-522025193138.jpg)
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0001.jpg "Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of Rat testis, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0002.jpg)
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/BMAL1-Antibody-4F5N3-Western-Blot-NBP3-16467-img0001.jpg "Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of various lysates using BMAL1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16467_rabbit-bmal1-mab-4f5n3-522025125450.jpg)
![Western Blot: IRF3 [p Ser396] Antibody [NBP3-05445] - Western blot analysis of extracts of NIH/3T3 cells, using IRF3 antibody (NBP3-05445) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/IRF3-[p-Ser396]-Antibody-Western-Blot-NBP3-05445-img0001.jpg "Western Blot: IRF3 [p Ser396] Antibody [NBP3-05445] - Western blot analysis of extracts of NIH/3T3 cells, using IRF3 antibody (NBP3-05445) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: IRF3 [p Ser396] Antibody - BSA Free [IRF3] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using IRF3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05445_rabbit-polyclonal-irf3-p-ser396-antibody-5220251638071.jpg)
![Western Blot: NRSF Antibody [NBP2-93006] - Western blot analysis of extracts of various cell lines, using NRSF antibody (NBP2-93006) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NRSF-Antibody-Western-Blot-NBP2-93006-img0001.jpg "Western Blot: NRSF Antibody [NBP2-93006] - Western blot analysis of extracts of various cell lines, using NRSF antibody (NBP2-93006) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: NRSF Antibody [NBP2-93006] - Western blot analysis of extracts of various cell lines, using NRSF antibody (NBP2-93006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/NRSF Antibody-Western Blot-NBP2-93006-img0002.jpg)
![Western Blot: NAC1 Antibody (6H2) [NBP1-51681] - Analysis using NACC1 mAb against HEK293 (1) and NACC1(AA: 165-438)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NAC1-Antibody-6H2-Western-Blot-NBP1-51681-img0005.jpg "Western Blot: NAC1 Antibody (6H2) [NBP1-51681] - Analysis using NACC1 mAb against HEK293 (1) and NACC1(AA: 165-438)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: NAC1 Antibody (6H2) [NBP1-51681] - Analysis of mammary cancer tissues (left) and ovarian cancer tissues (right) using NACC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NAC1-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51681-img0004.jpg)
![Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-278202416285256.jpg "Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Immunocytochemistry analysis of HeLa cells labeling DNMT1 with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-2920241192358.jpg)
![Western Blot: NFYA Antibody [NBP2-87907] - WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-87907-img0002.jpg "Western Blot: NFYA Antibody [NBP2-87907] - WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells")
![Immunohistochemistry: NFYA Antibody [NBP2-87907] - Human Liver](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87907-img0001.jpg)
![Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/YB1-Antibody-Western-Blot-NBP2-98908-img0003.jpg "Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa")
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP2-98908] - Immunofluorescence staining of YB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98908 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98908_rabbit-polyclonal-yb1-antibody-235202514331553.png)
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Western Blot: Menin Antibody (JE30-97) [NBP3-32588] - Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (NBP3-32588) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: PC-3M cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32588) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32588_rabbit-menin-mab-je30-97-27820241631650.png "Western Blot: Menin Antibody (JE30-97) [NBP3-32588] - Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (NBP3-32588) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: PC-3M cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32588) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Menin Antibody (JE30-97) [NBP3-32588] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Menin antibody (NBP3-32588) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32588) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32588_rabbit-menin-mab-je30-97-298202413585580.jpg)