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![Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.](http://images.novusbio.com/fullsize/p_6190_1.jpg "Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.")
![Chromatin Immunoprecipitation: KAT3B/p300 Antibody (1H11G2) [NBP2-59233] - ChIP was performed using HeLa cells, the antibody against p300 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-1H11G2-Chromatin-Immunoprecipitation-NBP2-59233-img0001.jpg "Chromatin Immunoprecipitation: KAT3B/p300 Antibody (1H11G2) [NBP2-59233] - ChIP was performed using HeLa cells, the antibody against p300 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Immunohistochemistry-Paraffin: Estrogen Receptor Antibody (S21-V) [NBP1-30153] - human of breast tumor tissue stained with Estrogen Receptor antibody shows strong positive nuclear immunostaining.](http://images.novusbio.com/fullsize/108721_1.jpg "Immunohistochemistry-Paraffin: Estrogen Receptor Antibody (S21-V) [NBP1-30153] - human of breast tumor tissue stained with Estrogen Receptor antibody shows strong positive nuclear immunostaining.")
![ELISA: NKX3.1 Antibody (3C1) [H00004824-M03] - Detection limit for recombinant GST tagged NKX3-1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-3C1-ELISA-H00004824-M03-img0001.jpg "ELISA: NKX3.1 Antibody (3C1) [H00004824-M03] - Detection limit for recombinant GST tagged NKX3-1 is approximately 1ng/ml as a capture antibody.")
![Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on coverslips stained with NBP3-45120 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-45120, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-45120_mouse-mhc-class-ii-mab-a6-61-3-bsa-free-immunocytochemistry-immunofluorescence-2220260322014.png "Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on coverslips stained with NBP3-45120 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-45120, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody ( isotype control, black line) or NBP3-45120 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-45120_mouse-mhc-class-ii-mab-a6-61-3-bsa-free-flow-cytometry-222026033311.png)
![Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272] - Immunofluorescence staining of fixed HeLa cells with NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of SAIC-26C-15 [NBP2-81272] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, most likely in the ER. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81272, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-SAIC-26C-15-Immunocytochemistry-Immunofluorescence-NBP2-81272-img0001.jpg "Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272] - Immunofluorescence staining of fixed HeLa cells with NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of SAIC-26C-15 [NBP2-81272] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, most likely in the ER. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81272, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: PKC zeta [p Thr560] Antibody (1B10) [NBP3-26399] - Positive Western Blot detected in A549 whole cell lysate. All lanes: PKC zeta [p Thr560] Antibody at 1.55ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68 KDa Observed band size: 68 KDa](http://images.novusbio.com/fullsize/nbp3-26399_rabbit-pkc-zeta-p-thr-560-mab-1b10-282202411472121.jpg "Western Blot: PKC zeta [p Thr560] Antibody (1B10) [NBP3-26399] - Positive Western Blot detected in A549 whole cell lysate. All lanes: PKC zeta [p Thr560] Antibody at 1.55ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68 KDa Observed band size: 68 KDa")