| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61769-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61897-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61896-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35645-20ul | |||
![Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129] - Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 ug per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 (R) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NBP2-19129-img0003.jpg "Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129] - Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 ug per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 (R) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.")
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NBP2-19129] - Rabbit anti STAT3 pY705 Antibody at 40X in colon tissue at pH 9. Tissue: Human Colon.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Immunohistochemistry-Paraffin-NBP2-19129-img0001.jpg)
![Immunohistochemistry of Rabbit anti STAT3 [p Tyr705] Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/nbp2-19129_rabbit-polyclonal-stat3-p-tyr705-antibody-235202318143127.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518395418.jpg "<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.mtTFA stained by mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-immunohistochemistry-paraffin-141120251839548.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518322913.jpg)
![Western Blot: EEF1A2 Antibody [NBP1-55245] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:12500 Positive Control: Human brain](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-55245-img0007.jpg "Western Blot: EEF1A2 Antibody [NBP1-55245] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:12500 Positive Control: Human brain")
![Western Blot: EEF1A2 Antibody [NBP1-55245] - Antibody Titration: 1 ug/ml Human HepG2.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-55245-img0004.jpg)
![Western Blot: EEF1A2 Antibody [NBP1-55245] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-55245-img0005.jpg)
![Western Blot: EEF1A2 Antibody [NBP1-55245] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-55245-img0006.jpg)
![Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-59164-img0001.jpg "Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: OCT4 Antibody [NBP2-59164] - ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the antibody against OCT4 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/OCT4-Antibody-Chromatin-Immunoprecipitation-NBP2-59164-img0003.jpg)
![ELISA: OCT4 Antibody [NBP2-59164] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against OCT4. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100,000.](http://images.novusbio.com/fullsize/OCT4-Antibody-ELISA-NBP2-59164-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0003.jpg "Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)")
![Flow Cytometry: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Flow-Cytometry-NBP2-61769-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0004.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-ELISA-NBP2-61769-img0001.jpg)
![Western Blot: TCF4 Antibody (7B1B3) [NBP2-61897] - Analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)](http://images.novusbio.com/fullsize/TCF4-Antibody-7B1B3-Western-Blot-NBP2-61897-img0003.jpg "Western Blot: TCF4 Antibody (7B1B3) [NBP2-61897] - Analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)")
![Flow Cytometry: TCF4 Antibody (7B1B3) [NBP2-61897] - Analysis of Hela cells using TCF4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TCF4-Antibody-7B1B3-Flow-Cytometry-NBP2-61897-img0002.jpg)
![Western Blot: TCF4 Antibody (7B1B3) [NBP2-61897] - Analysis using TCF4 mAb against HEK293 (1) and TCF4 (AA: 518-667)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TCF4-Antibody-7B1B3-Western-Blot-NBP2-61897-img0004.jpg)
![ELISA: TCF4 Antibody (7B1B3) [NBP2-61897] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TCF4-Antibody-7B1B3-ELISA-NBP2-61897-img0001.jpg)
![Western Blot: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)](http://images.novusbio.com/fullsize/TCF4-Antibody-4D4C4-Western-Blot-NBP2-61896-img0003.jpg "Western Blot: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)")
![Flow Cytometry: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis of K562 cells using TCF4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TCF4-Antibody-4D4C4-Flow-Cytometry-NBP2-61896-img0002.jpg)
![Western Blot: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis using TCF4 mAb against HEK293 (1) and TCF4 (AA: 518-667)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TCF4-Antibody-4D4C4-Western-Blot-NBP2-61896-img0004.jpg)
![ELISA: TCF4 Antibody (4D4C4) [NBP2-61896] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TCF4-Antibody-4D4C4-ELISA-NBP2-61896-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: HEY1 Antibody [NBP3-35645] - Immunofluorescence analysis of A431 cells using HEY1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35645_rabbit-hey1-pab-1012202411531090.jpg "Immunocytochemistry/ Immunofluorescence: HEY1 Antibody [NBP3-35645] - Immunofluorescence analysis of A431 cells using HEY1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: HEY1 Antibody [NBP3-35645] - Western blot analysis of various lysates using HEY1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35645_rabbit-hey1-pab-101220241233830.jpg)