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![Western Blot:[NBP3-29680]-Detection of human and mouse SUCLA2 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUCLA2 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29680_rabbit-sucla2-pab-231202512884.jpg "Western Blot:[NBP3-29680]-Detection of human and mouse SUCLA2 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUCLA2 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:DPP3 AntibodyNBP3-29845] -Detection of human DPP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DPP3 antibody NBP3-29845 (lot NBP3-29845-1) used for IP at 6 µg per reaction. DPP3 was also immunoprecipitated by rabbit anti-DPP3.. For blotting immunoprecipitated DPP3, NBP3-29845 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29845_rabbit-dpp3-pab-301220242041575.jpg "Immunoprecipitation:DPP3 AntibodyNBP3-29845] -Detection of human DPP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DPP3 antibody NBP3-29845 (lot NBP3-29845-1) used for IP at 6 µg per reaction. DPP3 was also immunoprecipitated by rabbit anti-DPP3.. For blotting immunoprecipitated DPP3, NBP3-29845 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249342744.jpg "Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249374967.jpg)
![Immunoprecipitation:COX5b AntibodyNBP3-30407] -Detection of human COX5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5B antibody NBP3-30407 (lot NBP3-30407-1) used for IP at 6 µg per reaction. COX5B was also immunoprecipitated by rabbit anti-COX5B.. For blotting immunoprecipitated COX5B, NBP3-30407 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30407_rabbit-cox5b-pab-3012202412262516.jpg "Immunoprecipitation:COX5b AntibodyNBP3-30407] -Detection of human COX5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5B antibody NBP3-30407 (lot NBP3-30407-1) used for IP at 6 µg per reaction. COX5B was also immunoprecipitated by rabbit anti-COX5B.. For blotting immunoprecipitated COX5B, NBP3-30407 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:HNF-3 alpha/FoxA1 AntibodyNBP3-29853] -Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody NBP3-29853 (lot NBP3-29853-1) used for IP at 6 µg per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, NBP3-29853 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29853_rabbit-hnf-3-alpha-foxa1-pab-301220241727488.jpg "Immunoprecipitation:HNF-3 alpha/FoxA1 AntibodyNBP3-29853] -Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody NBP3-29853 (lot NBP3-29853-1) used for IP at 6 µg per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, NBP3-29853 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:COX5b AntibodyNBP3-30406] -Detection of human COX5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5B antibody NBP3-30406 (lot NBP3-30406-1) used for IP at 6 µg per reaction. COX5B was also immunoprecipitated by rabbit anti-COX5B.. For blotting immunoprecipitated COX5B, NBP3-30406 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30406_rabbit-cox5b-pab-301220241710130.jpg "Immunoprecipitation:COX5b AntibodyNBP3-30406] -Detection of human COX5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5B antibody NBP3-30406 (lot NBP3-30406-1) used for IP at 6 µg per reaction. COX5B was also immunoprecipitated by rabbit anti-COX5B.. For blotting immunoprecipitated COX5B, NBP3-30406 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry:[NBP3-29223]-Detection of human NCBP2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NCBP2.](http://images.novusbio.com/fullsize/nbp3-29223_rabbit-cbp20-pab-231202511595090.jpg "Immunohistochemistry:[NBP3-29223]-Detection of human NCBP2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NCBP2.")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded Mouse Prostate stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-11-Immunohistochemistry-Paraffin-NBP3-07431-img0002.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded Mouse Prostate stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded human Prostate Cancer stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-11-Immunohistochemistry-Paraffin-NBP3-07431-img0001.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-1411202518392011.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-14112025184051.jpg)
![Immunoprecipitation:[NBP3-29021]-Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated RPSA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29021_rabbit-rpsa-pab-231202512101681.jpg "Immunoprecipitation:[NBP3-29021]-Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated RPSA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:GRP75/HSPA9B/Mortalin AntibodyNBP3-29778] -Detection of human HSPA9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSPA9 antibody NBP3-29778 (lot NBP3-29778-1) used for IP at 6 µg per reaction. HSPA9 was also immunoprecipitated by rabbit anti-HSPA9 antibody. for blotting immunoprecipitated HSPA9, NBP3-29778 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29778_rabbit-grp75-hspa9b-mortalin-pab-301220241536292.jpg "Immunoprecipitation:GRP75/HSPA9B/Mortalin AntibodyNBP3-29778] -Detection of human HSPA9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSPA9 antibody NBP3-29778 (lot NBP3-29778-1) used for IP at 6 µg per reaction. HSPA9 was also immunoprecipitated by rabbit anti-HSPA9 antibody. for blotting immunoprecipitated HSPA9, NBP3-29778 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunoprecipitation:Isoleucyl tRNA synthetase AntibodyNBP3-30404] -Detection of human IARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IARS antibody NBP3-30404 (lot NBP3-30404-1) used for IP at 6 µg per reaction. IARS was also immunoprecipitated by rabbit anti-IARS antibody . For blotting immunoprecipitated IARS, NBP3-30404 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30404_rabbit-isoleucyl-trna-synthetase-pab-301220241828469.jpg "Immunoprecipitation:Isoleucyl tRNA synthetase AntibodyNBP3-30404] -Detection of human IARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IARS antibody NBP3-30404 (lot NBP3-30404-1) used for IP at 6 µg per reaction. IARS was also immunoprecipitated by rabbit anti-IARS antibody . For blotting immunoprecipitated IARS, NBP3-30404 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")