| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99560-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90054-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97173-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89882-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98988-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98603-50ul | |||
![Western Blot: ATP5O Antibody [NBP2-99560] - Anti-ATP5O rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: COS7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa](http://images.novusbio.com/fullsize/ATP5O-Antibody-Western-Blot-NBP2-99560-img0004.jpg "Western Blot: ATP5O Antibody [NBP2-99560] - Anti-ATP5O rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: COS7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa")
![Immunohistochemistry-Paraffin: ATP5O Antibody [NBP2-99560] - Immunochemical staining of human ATP5O in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5O-Antibody-Immunohistochemistry-Paraffin-NBP2-99560-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5O Antibody [NBP2-99560] - Immunochemical staining of human ATP5O in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5O-Antibody-Immunohistochemistry-Paraffin-NBP2-99560-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (001) [NBP2-90054] - Staining of human FABP4 in human appendix with rabbit monoclonal antibody (1:2000).](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90054-img0002.jpg "Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (001) [NBP2-90054] - Staining of human FABP4 in human appendix with rabbit monoclonal antibody (1:2000).")
![Flow Cytometry: FABP4/A-FABP Antibody (001) [NBP2-90054] - Analysis of Human FABP4 expression on Hela cells. The cells were treated, stained with purified anti-Human FABP4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-001-Flow-Cytometry-NBP2-90054-img0003.jpg)
![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (001) [NBP2-90054] - Staining of human FABP4 in human lung cancer with rabbit monoclonal antibody (1:2000).](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90054-img0001.jpg)
![Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97173-img0003.jpg "Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97173-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FLRT1 Antibody [NBP2-97173] - Immunofluorescence staining of FLRT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97173 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-97173_rabbit-polyclonal-flrt1-antibody-235202511444487.png)
![Western Blot: AKR1B1 Antibody (109) [NBP2-89882] - Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-109-Western-Blot-NBP2-89882-img0001.jpg "Western Blot: AKR1B1 Antibody (109) [NBP2-89882] - Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa")
![Immunoprecipitation: AKR1B1 Antibody (109) [NBP2-89882] - Lane A:0.5 mg Jurkat Whole Cell Lysate 0.5 uL anti-AKR1B1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AKR1B1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-109-Immunoprecipitation-NBP2-89882-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (109) [NBP2-89882] - Staining of Human AKR1B1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89882 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-89882_rabbit-monoclonal-akr1b1-antibody-109-235202514492367.png)
![Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-278202415242624.jpg "Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32638) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-298202414115422.jpg)
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0003.jpg "Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CDCA3 Antibody [NBP2-98988] - Immunofluorescence staining of CDCA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98988 antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98988_rabbit-polyclonal-cdca3-antibody-235202521451716.png)
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-298202414333631.jpg "Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-29820241435244.jpg)
![Immunocytochemistry/ Immunofluorescence: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunocytochemistry analysis of 293T cells labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-2920241192312.jpg)
![Western Blot: Synapsin 3 Antibody [NBP2-98603] - Lane A: NCIH460 Whole Cell LysateLane B: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:63 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Synapsin-3-Antibody-Western-Blot-NBP2-98603-img0004.jpg "Western Blot: Synapsin 3 Antibody [NBP2-98603] - Lane A: NCIH460 Whole Cell LysateLane B: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:63 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: Synapsin 3 Antibody [NBP2-98603] - Staining of human Synapsin 3 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Synapsin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-98603-img0003.jpg)
![Immunohistochemistry-Paraffin: Synapsin 3 Antibody [NBP2-98603] - Staining of human Synapsin 3 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Synapsin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-98603-img0002.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-288202412442922.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-29202410484847.jpg)
![Flow Cytometry: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Flow cytometric analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32768, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-2920241637217.jpg)
![Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Raji cell lysate (20 ug/Lane) Lane 3: Ramos cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: 293T cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse thymus tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat thymus tissue lysate (40 ug/Lane) Lane 13: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32223_rabbit-crlf3-mab-psh03-88-278202415212426.jpg "Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Raji cell lysate (20 ug/Lane) Lane 3: Ramos cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: 293T cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse thymus tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat thymus tissue lysate (40 ug/Lane) Lane 13: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32223_rabbit-crlf3-mab-psh03-88-29202410234273.jpg)
![Western Blot: SNX5 Antibody (JE64-96) [NBP3-32965] - Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32965) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32965_rabbit-snx5-mab-je64-96-288202412493454.jpg "Western Blot: SNX5 Antibody (JE64-96) [NBP3-32965] - Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32965) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNX5 Antibody (JE64-96) [NBP3-32965] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32965) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32965_rabbit-snx5-mab-je64-96-29820241426261.jpg)