| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89220-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97782-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99267-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
![Immunohistochemistry-Paraffin: OXA1L Antibody [NBP2-97443] - Immunochemical staining of human OXA1L in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/OXA1L-Antibody-Immunohistochemistry-Paraffin-NBP2-97443-img0003.jpg "Immunohistochemistry-Paraffin: OXA1L Antibody [NBP2-97443] - Immunochemical staining of human OXA1L in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: OXA1L Antibody [NBP2-97443] - Immunochemical staining of human OXA1L in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/OXA1L-Antibody-Immunohistochemistry-Paraffin-NBP2-97443-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: OXA1L Antibody [NBP2-97443] - Immunofluorescence staining of OXA1L in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97443 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97443_rabbit-polyclonal-oxa1l-antibody-235202521451724.png)
![Immunohistochemistry-Paraffin: GDF-3 Antibody (010) [NBP2-89220] - Immunochemical staining of human GDF-3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GDF-3-Antibody-010-Immunohistochemistry-Paraffin-NBP2-89220-img0003.jpg "Immunohistochemistry-Paraffin: GDF-3 Antibody (010) [NBP2-89220] - Immunochemical staining of human GDF-3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: GDF-3 Antibody (010) [NBP2-89220] - Staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100).](http://images.novusbio.com/fullsize/GDF-3-Antibody-010-Immunohistochemistry-Paraffin-NBP2-89220-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: GDF-3 Antibody (010) [NBP2-89220] - Immunofluorescence staining of GDF-3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89220 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89220_rabbit-monoclonal-gdf-3-antibody-010-235202511354114.png)
![Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-288202412461665.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32810) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32810) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-298202414161831.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Western Blot: CRIPTO Antibody (JE33-31) [NBP3-32221] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32221) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-278202416285240.jpg "Western Blot: CRIPTO Antibody (JE33-31) [NBP3-32221] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32221) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody (JE33-31) [NBP3-32221] - Immunocytochemistry analysis of NCCIT cells labeling CRIPTO with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-2920241114828.jpg)
![Flow Cytometry: CRIPTO Antibody (JE33-31) [NBP3-32221] - Flow cytometric analysis of NCCIT cells labeling CRIPTO. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32221, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-392024884631.jpg)
![Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/RPA14-Antibody-Western-Blot-NBP2-97782-img0004.jpg "Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa")
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0003.jpg)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0002.jpg)
![Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-288202412442957.jpg "Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Immunocytochemistry analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes, then treated with lambda pp for 1 hour labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-29202410234248.jpg)
![Western Blot: DLX3 Antibody [NBP2-88802] - WB Suggested Anti-DLX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T](http://images.novusbio.com/fullsize/DLX3-Antibody-Western-Blot-NBP2-88802-img0003.jpg "Western Blot: DLX3 Antibody [NBP2-88802] - WB Suggested Anti-DLX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T")
![Immunohistochemistry: DLX3 Antibody [NBP2-88802] - Placenta](http://images.novusbio.com/fullsize/DLX3-Antibody-Immunohistochemistry-NBP2-88802-img0002.jpg)
![Immunohistochemistry: DLX3 Antibody [NBP2-88802] - Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/DLX3-Antibody-Immunohistochemistry-NBP2-88802-img0001.jpg)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-392024848501.jpg)
![Western Blot: CCT3 Antibody [NBP2-99267] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP2-99267-img0001.jpg "Western Blot: CCT3 Antibody [NBP2-99267] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa")
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP2-99267] - Immunochemical staining of human CCT3 in human prostate with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP2-99267-img0003.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP2-99267] - Immunochemical staining of human CCT3 in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP2-99267-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")