| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99236-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99340-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06643-50ul | |||
![Western Blot: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Western blot analysis of HNF-3 alpha/FoxA1 on LNCaP cell lysates with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-278202415212490.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Western blot analysis of HNF-3 alpha/FoxA1 on LNCaP cell lysates with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32346) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-298202414333671.jpg)
![Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Immunocytochemistry analysis of LNCaP cells labeling HNF-3 alpha/FoxA1 with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-2920241192352.jpg)
![Western Blot: FALDH Antibody [NBP2-99236] - Lane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:54 kDa](http://images.novusbio.com/fullsize/FALDH-Antibody-Western-Blot-NBP2-99236-img0004.jpg "Western Blot: FALDH Antibody [NBP2-99236] - Lane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:54 kDa")
![Immunohistochemistry-Paraffin: FALDH Antibody [NBP2-99236] - Staining of mouse FALDH in mouse liver with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/FALDH-Antibody-Immunohistochemistry-Paraffin-NBP2-99236-img0003.jpg)
![Immunoprecipitation: FALDH Antibody [NBP2-99236] - Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-Aldh3a2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Aldh3a2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 53 kDa](http://images.novusbio.com/fullsize/FALDH-Antibody-Immunoprecipitation-NBP2-99236-img0005.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Western Blot: Albumin Antibody (AL-01) [NB500-417] - Analysis (reducing conditions) of human serum albumin using anti-human Albumin (AL-01). Lane 1: Human blood plasma Lane 2: Human blood serum.](http://images.novusbio.com/fullsize/Albumin-Antibody-AL-01-Western-Blot-NB500-417-img0001.jpg "Western Blot: Albumin Antibody (AL-01) [NB500-417] - Analysis (reducing conditions) of human serum albumin using anti-human Albumin (AL-01). Lane 1: Human blood plasma Lane 2: Human blood serum.")
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-99340-img0005.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Immunochemical staining of human Pyruvate Dehydrogenase E2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-99340-img0003.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Pyruvate Dehydrogenase E2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunoprecipitation-NBP2-99340-img0004.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-27820241631646.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-29820241492678.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-2920241192361.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-278202415141712.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Actin Alpha 1 Cardiac Muscle with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32032, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420918.jpg)
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32032) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420938.jpg)
![Immunohistochemistry: DACH1 Antibody [NBP3-06643] - Immunohistochemical staining of human DACH1 in human kidney with NBP3-06643 at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/DACH1-Antibody-Immunohistochemistry-NBP3-06643-img0003.jpg "Immunohistochemistry: DACH1 Antibody [NBP3-06643] - Immunohistochemical staining of human DACH1 in human kidney with NBP3-06643 at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry: DACH1 Antibody [NBP3-06643] - Immunochemical staining of human DACH1 in human small intestine with NBP3-06643 at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/DACH1-Antibody-Immunohistochemistry-NBP3-06643-img0001.jpg)
![Immunohistochemistry: DACH1 Antibody [NBP3-06643] - Immunochemical staining of human DACH1 in human gallbladder with NBP3-06643 at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/DACH1-Antibody-Immunohistochemistry-NBP3-06643-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DACH1 Antibody [NBP3-06643] - Immunofluorescence staining of DACH1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-06643 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06643_rabbit-polyclonal-dach1-antibody-235202516144334.png)
![Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293] - Western blot analysis of eEF-2 on different lysates with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse liver tissue lysate (40 ug/Lane) Lane 6: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32293) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-278202416285245.jpg "Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293] - Western blot analysis of eEF-2 on different lysates with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse liver tissue lysate (40 ug/Lane) Lane 6: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32293) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (JE32-57) [NBP3-32293] - Immunocytochemistry analysis of HeLa cells labeling eEF-2 with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-29202410334013.jpg)
![Flow Cytometry: eEF-2 Antibody (JE32-57) [NBP3-32293] - Flow cytometric analysis of HeLa cells labeling eEF-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32293, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-29202416453236.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-MEM-189-Flow-Cytometry-NB500-493-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.")