| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35052-1mg | |||
| Carrier-Free | NBP2-35052-100ug | |||
| Carrier-Free | NBP2-35052-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37317-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97446-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06479-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97091-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00348-50ul | |||
![SDS-Page: Human FGF acidic/FGF1 Protein [NBP2-35052]](http://images.novusbio.com/fullsize/Human-FGF-acidic-FGF1-Protein-SDS-Page-NBP2-35052-img0001.jpg "SDS-Page: Human FGF acidic/FGF1 Protein [NBP2-35052]")
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mouse mAb against Jurkat (1), Hela (2), K562 (3), Cos7 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), HEK293 (8), RAJI (9) cell lysate.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0004.jpg "Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mouse mAb against Jurkat (1), Hela (2), K562 (3), Cos7 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), HEK293 (8), RAJI (9) cell lysate.")
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mAb against human TFRC recombinant protein. (Expected MW is 39.7 kDa)](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0002.jpg)
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mAb against HEK293 (1) and TFRC (AA: 608-727)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0003.jpg)
![ELISA: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-ELISA-NBP2-37317-img0001.jpg)
![Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 .](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-74496-img0002.jpg "Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 .")
![Immunohistochemistry: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74496-img0003.jpg)
![Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of extracts (10ug) from 2 different cell lines.](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-74496-img0001.jpg)
![Immunohistochemistry: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74496-img0004.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0005.jpg "Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).")
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0003.jpg)
![Western Blot: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:57 kDa](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Western-Blot-NBP3-06479-img0001.jpg "Western Blot: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:57 kDa")
![Immunohistochemistry-Paraffin: UDP glucose dehydrogenase Antibody [NBP3-06479] - Staining of human UGDH in human prostate with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP3-06479-img0003.jpg)
![Immunohistochemistry-Paraffin: UDP glucose dehydrogenase Antibody [NBP3-06479] - Staining of human UGDH in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP3-06479-img0002.jpg)
![Immunoprecipitation: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-UGDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UGDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :57 kDa](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunoprecipitation-NBP3-06479-img0004.jpg)
![Western Blot: COX5b Antibody [NBP2-97091] - Anti-COX5b rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 13 kDa](http://images.novusbio.com/fullsize/COX5b-Antibody-Western-Blot-NBP2-97091-img0006.jpg "Western Blot: COX5b Antibody [NBP2-97091] - Anti-COX5b rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 13 kDa")
![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0004.jpg)
![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0003.jpg)
![Immunoprecipitation: COX5b Antibody [NBP2-97091] - COX5b was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-COX5b rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-COX5b rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunoprecipitation-NBP2-97091-img0005.jpg)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-2352025162819.png "Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa")
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545641.png)
![Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-23520251664731.png)
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545651.png)
![Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-278202415212451.jpg "Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H2-R) [NBP3-32043] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-298202414333696.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H2-R) [NBP3-32043] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-2920241192367.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Western blot analysis of PP2C gamma/PPM1G on different lysates with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/500 dilution. Lane 1: 293T cell lysate, 10 ug/Lane Lane 2: Jurkat cell lysate, 10 ug/Lane Lane 3: Rat testis tissue lysate, 20 ug/Lane Predicted band size: 59 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32844) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-288202412461628.jpg "Western Blot: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Western blot analysis of PP2C gamma/PPM1G on different lysates with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/500 dilution. Lane 1: 293T cell lysate, 10 ug/Lane Lane 2: Jurkat cell lysate, 10 ug/Lane Lane 3: Rat testis tissue lysate, 20 ug/Lane Predicted band size: 59 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32844) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32844) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-2982024142091.jpg)
![Flow Cytometry: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Flow cytometric analysis of HepG2 cells labeling PP2C gamma/PPM1G. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32844, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-392024884672.jpg)