| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03693-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04365-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37288-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37362-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00055-50ul | |||
![Western Blot: Laforin/EPM2A Antibody [NBP2-81945] - Analysis of Laforin/EPM2A in SW480 cell lysate with Laforin/EPM2A antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Laforin-EPM2A-Antibody-Western-Blot-NBP2-81945-img0001.jpg "Western Blot: Laforin/EPM2A Antibody [NBP2-81945] - Analysis of Laforin/EPM2A in SW480 cell lysate with Laforin/EPM2A antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Laforin/EPM2A Antibody [NBP2-81945] - Analysis of Laforin/EPM2A in SW480 cells with Laforin/EPM2A antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Laforin-EPM2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81945-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Laforin/EPM2A Antibody - BSA Free [NBP2-81945] - Immunofluorescence of Laforin/EPM2A in SW480 cells with Laforin/EPM2A antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81945_rabbit-polyclonal-laforin-epm2a-antibody-2711202420441148.jpg)
![Western Blot: pan PKC Antibody [NBP3-05673] - Analysis of extracts of various cell lines, using pan PKC antibody (NBP3-05673) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/pan-PKC-Antibody-Western-Blot-NBP3-05673-img0002.jpg "Western Blot: pan PKC Antibody [NBP3-05673] - Analysis of extracts of various cell lines, using pan PKC antibody (NBP3-05673) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: pan PKC Antibody [NBP3-05673] - Immunofluorescence analysis of NIH-3T3 cells using pan PKC Rabbit pAb (NBP3-05673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/pan-PKC-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05673-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: pan PKC Antibody - BSA Free [pan PKC] - Immunofluorescence analysis of NIH-3T3 cells using pan PKC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05673_rabbit-polyclonal-pan-pkc-antibody-5220251726080.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241252814.jpg "Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: PTP epsilon Antibody [NBP3-38398] - Western blot analysis of various lysates using PTP epsilon Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-1012202411531092.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241224188.jpg)
![Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/STEP-Antibody-Western-Blot-NBP3-03693-img0001.jpg "Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of Neuro-2a cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-52202519302959.jpg)
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of SH-SY5Y cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-5220251931349.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
![Western Blot: EYA1 Antibody [NBP3-04365] - Western blot analysis of extracts of various cell lines, using EYA1 Rabbit pAb (NBP3-04365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/EYA1-Antibody-Western-Blot-NBP3-04365-img0003.jpg "Western Blot: EYA1 Antibody [NBP3-04365] - Western blot analysis of extracts of various cell lines, using EYA1 Rabbit pAb (NBP3-04365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: EYA1 Antibody [NBP3-04365] - Immunohistochemistry of paraffin-embedded Rat brain using EYA1 Rabbit pAb (NBP3-04365) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/EYA1-Antibody-Immunohistochemistry-Paraffin-NBP3-04365-img0002.jpg)
![Immunohistochemistry-Paraffin: EYA1 Antibody [NBP3-04365] - Immunohistochemistry of paraffin-embedded Human placenta using EYA1 Rabbit pAb (NBP3-04365) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/EYA1-Antibody-Immunohistochemistry-Paraffin-NBP3-04365-img0001.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0003.jpg "Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Flow cytometric analysis of Hela cells using PPP1R1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Flow-Cytometry-NBP2-37288-img0004.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0002.jpg)
![ELISA: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-ELISA-NBP2-37288-img0001.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Western blot analysis using ACP5 mouse mAb against JURKAT (1) and OCM-1 (2) cell lysate.](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-5C5E7-Western-Blot-NBP2-37362-img0004.jpg "Western Blot: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Western blot analysis using ACP5 mouse mAb against JURKAT (1) and OCM-1 (2) cell lysate.")
![Flow Cytometry: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Flow cytometric analysis of JURKAT cells using ACP5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-5C5E7-Flow-Cytometry-NBP2-37362-img0005.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Western blot analysis using ACP5 mAb against human ACP5 recombinant protein. (Expected MW is 37.3 kDa)](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-5C5E7-Western-Blot-NBP2-37362-img0002.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Western blot analysis using ACP5 mAb against HEK293 (1) and ACP5 (AA: 221-325)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-5C5E7-Western-Blot-NBP2-37362-img0003.jpg)
![ELISA: TRACP/PAP/ACP5 Antibody (5C5E7) [NBP2-37362] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-5C5E7-ELISA-NBP2-37362-img0001.jpg)
![Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-288202412442941.png "Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32950) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-298202413585563.jpg)
![Flow Cytometry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Flow cytometric analysis of THP-1 cells labeling SIRP alpha/CD172a. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32950, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-2920241637213.png)
![Western Blot: PTPMT1 Antibody [NBP3-00055] - Anti-PTPMT1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 18 kDa](http://images.novusbio.com/fullsize/PTPMT1-Antibody-Western-Blot-NBP3-00055-img0005.jpg "Western Blot: PTPMT1 Antibody [NBP3-00055] - Anti-PTPMT1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 18 kDa")
![Immunohistochemistry-Paraffin: PTPMT1 Antibody [NBP3-00055] - Immunochemical staining of human PTPMT1 in human pancreatic carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PTPMT1-Antibody-Immunohistochemistry-Paraffin-NBP3-00055-img0004.jpg)
![Immunohistochemistry-Paraffin: PTPMT1 Antibody [NBP3-00055] - Immunochemical staining of human PTPMT1 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PTPMT1-Antibody-Immunohistochemistry-Paraffin-NBP3-00055-img0002.jpg)
![Immunohistochemistry-Paraffin: PTPMT1 Antibody [NBP3-00055] - Immunochemical staining of human PTPMT1 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PTPMT1-Antibody-Immunohistochemistry-Paraffin-NBP3-00055-img0003.jpg)
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] -Analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 88 kDaObserved band size: 100 kDaExposure time: 5 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66844_rabbit-monoclonal-fibroblast-activation-protein-alpha-fap-antibody-ja56-11-western-blot-127202491739..jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] -Analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 88 kDaObserved band size: 100 kDaExposure time: 5 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FAP antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-66844_rabbit-monoclonal-fibroblast-activation-protein-alpha-fap-antibody-ja56-11-immunohistochemistry-paraffin-127202491856..jpg)
![Western Blot: PNK Antibody (JE64-21) [NBP3-32835] - Western blot analysis of PNK on different lysates with Rabbit anti-PNK antibody (NBP3-32835) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32835) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-288202412442958.jpg "Western Blot: PNK Antibody (JE64-21) [NBP3-32835] - Western blot analysis of PNK on different lysates with Rabbit anti-PNK antibody (NBP3-32835) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32835) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PNK Antibody (JE64-21) [NBP3-32835] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PNK antibody (NBP3-32835) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32835) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-298202414333612.jpg)
![Immunocytochemistry/ Immunofluorescence: PNK Antibody (JE64-21) [NBP3-32835] - Immunocytochemistry analysis of Hela cells labeling PNK with Rabbit anti-PNK antibody (NBP3-32835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PNK antibody (NBP3-32835) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-2920241192375.jpg)