| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90037-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98761-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99118-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98726-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
![Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765] - Western blot analysis of ErbB3/Her3 on different lysates with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 148 kDa Observed band size: 180 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32765_rabbit-erbb3-her3-p-tyr1289-mab-je51-25-288202412461646.jpg "Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765] - Western blot analysis of ErbB3/Her3 on different lysates with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 148 kDa Observed band size: 180 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765] - Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling ErbB3/Her3 with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32765_rabbit-erbb3-her3-p-tyr1289-mab-je51-25-29202410234297.jpg)
![Western Blot: Myt1 Antibody [NBP2-87873] - WB Suggested Anti-MYT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate](http://images.novusbio.com/fullsize/Myt1-Antibody-Western-Blot-NBP2-87873-img0002.jpg "Western Blot: Myt1 Antibody [NBP2-87873] - WB Suggested Anti-MYT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate")
![Immunohistochemistry-Paraffin: Myt1 Antibody [NBP2-87873] - Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage cells. Dilution: 1:1000](http://images.novusbio.com/fullsize/Myt1-Antibody-Immunohistochemistry-Paraffin-NBP2-87873-img0001.jpg)
![Flow Cytometry: PDGF R beta Antibody (18A2) [NBP1-47232] - Human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) APC.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-18A2-Flow-Cytometry-NBP1-47232-img0002.jpg "Flow Cytometry: PDGF R beta Antibody (18A2) [NBP1-47232] - Human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) APC.")
![Flow Cytometry: PDGF R beta Antibody (18A2) [NBP1-47232] - Human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) purified GAM-APC.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-18A2-Flow-Cytometry-NBP1-47232-img0003.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0003.jpg "Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human melanoma with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0001.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human skin with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0002.jpg)
![Western Blot: ROR2 Antibody (JE37-72) [NBP3-32916] - Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (NBP3-32916) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32916) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32916_rabbit-ror2-mab-je37-72-288202412442985.jpg "Western Blot: ROR2 Antibody (JE37-72) [NBP3-32916] - Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (NBP3-32916) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32916) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROR2 Antibody (JE37-72) [NBP3-32916] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ROR2 antibody (NBP3-32916) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32916) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32916_rabbit-ror2-mab-je37-72-29820241420986.jpg)
![Western Blot: PKN1 Antibody [NBP2-98761] - Lane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:125 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-98761_rabbit-polyclonal-pkn1-antibody-western-blot-229202394441..jpg "Western Blot: PKN1 Antibody [NBP2-98761] - Lane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:125 kDa")
![Immunocytochemistry/Immunofluorescence: PKN1 Antibody [NBP2-98761] - Immunofluorescence staining of PKN1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98761 antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98761_rabbit-polyclonal-pkn1-antibody-23520251125447.png)
![Immunohistochemistry-Paraffin: CABC1 Antibody [NBP2-99118] - Immunochemical staining of human CABC1 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CABC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99118-img0002.jpg "Immunohistochemistry-Paraffin: CABC1 Antibody [NBP2-99118] - Immunochemical staining of human CABC1 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: CABC1 Antibody [NBP2-99118] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:72 kDaObserved band size:72 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99118_rabbit-polyclonal-cabc1-antibody-western-blot-2482023104735..jpg)
![Western Blot: PTF1A Antibody [NBP2-98726] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PTF1A-Antibody-Western-Blot-NBP2-98726-img0003.jpg "Western Blot: PTF1A Antibody [NBP2-98726] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: PTF1A Antibody [NBP2-98726] - Immunofluorescence staining of PTF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98726 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98726_rabbit-polyclonal-ptf1a-antibody-23520252135184.png)
![Western Blot: RIPK3/RIP3 Antibody (PSH04-78) [NBP3-32905] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32905) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32905_rabbit-ripk3-rip3-mab-psh04-78-288202412461621.jpg "Western Blot: RIPK3/RIP3 Antibody (PSH04-78) [NBP3-32905] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32905) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RIPK3/RIP3 Antibody (PSH04-78) [NBP3-32905] - Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32905) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32905) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32905_rabbit-ripk3-rip3-mab-psh04-78-298202414333611.jpg)
![Western Blot: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32906) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32906_rabbit-ripk3-rip3-mab-psh04-79-288202412461630.jpg "Western Blot: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32906) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32906) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32906_rabbit-ripk3-rip3-mab-psh04-79-298202413585590.jpg)
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-29202410484840.jpg "Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: 293T cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: MCF7 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-3920248485023.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)