| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26455-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26212-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26348-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26619-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26397-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26251-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26378-50ul | |||
![Western Blot: TrkA Antibody (2A5) [NBP3-26455] - Positive Western Blot detected in: Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: TrkA Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 87, 84, 78 kDa Observed band size: 145 kDa](http://images.novusbio.com/fullsize/nbp3-26455_rabbit-trka-mab-2a5-28220241140937.jpg "Western Blot: TrkA Antibody (2A5) [NBP3-26455] - Positive Western Blot detected in: Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: TrkA Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 87, 84, 78 kDa Observed band size: 145 kDa")
![Immunoprecipitation: Girdin Antibody [NBP1-71856] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71857 , which recognizes a downstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71856-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: Girdin Antibody [NBP1-71856] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71857 , which recognizes a downstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.")
![Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/STAT1-[p-Tyr701]-Antibody-Western-Blot-NBP1-77825-img0001.jpg "Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.")
![Western Blot: KIDINS220 Antibody [NBP1-50034] - Detection of Human ARMS by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ARMS antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes a downstream epitope. For blotting immunoprecipitated ARMS, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/KIDINS220-Antibody-Western-Blot-NBP1-50034-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: KIDINS220 Antibody [NBP1-50034] - Detection of Human ARMS by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ARMS antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes a downstream epitope. For blotting immunoprecipitated ARMS, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry: Huntingtin Antibody (6F2) [NBP3-26212] - Image of Huntingtin Antibody (6F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26212_rabbit-huntingtin-mab-6f2-262202415355726.jpg "Immunohistochemistry: Huntingtin Antibody (6F2) [NBP3-26212] - Image of Huntingtin Antibody (6F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Huntingtin Antibody (6F2) [NBP3-26212] - Image of Huntingtin Antibody (6F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26212_rabbit-huntingtin-mab-6f2-2722024827494.jpg)
![Western Blot: KIDINS220 Antibody [NBP1-50035] - Detection of Human ARMS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARMS antibod used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes an upstream epitope. For blotting immunoprecipitated ARMS was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KIDINS220-Antibody-Western-Blot-NBP1-50035-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: KIDINS220 Antibody [NBP1-50035] - Detection of Human ARMS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARMS antibod used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes an upstream epitope. For blotting immunoprecipitated ARMS was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: RelB Antibody (9E4) [NBP3-26348] - Staining of Hela with RelB Antibody (9E4) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 507-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26348_rabbit-relb-mab-9e4-28220241312215.jpg "Immunocytochemistry/Immunofluorescence: RelB Antibody (9E4) [NBP3-26348] - Staining of Hela with RelB Antibody (9E4) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 507-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: RelB Antibody (9E4) [NBP3-26348] - Overlay Peak curve showing Hela cells stained with RelB Antibody (9E4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26348_rabbit-relb-mab-9e4-28220241418360.jpg)
![Western Blot: PSD-95 Antibody (1H4) [NBP3-26619] - Positive Western Blot detected in: Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: PSD-95 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81, 86, 81 kDa Observed band size: 95 kDa](http://images.novusbio.com/fullsize/nbp3-26619_rabbit-psd-95-mab-1h4-282202411442612.jpg "Western Blot: PSD-95 Antibody (1H4) [NBP3-26619] - Positive Western Blot detected in: Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: PSD-95 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81, 86, 81 kDa Observed band size: 95 kDa")
![Western Blot: Cdk5 Antibody (3#) [NBP3-41112] - Sample: Recombinant CDK5, Human.](http://images.novusbio.com/fullsize/nbp3-41112_mouse-cdk5-mab-3-612202421351236.jpg "Western Blot: Cdk5 Antibody (3#) [NBP3-41112] - Sample: Recombinant CDK5, Human.")
![Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Immunoprecipitating SHP-2/PTPN11 [p Tyr542] in Hela whole cell lysate treated with Pervanadate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26397 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26397 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg). Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26397_rabbit-shp-2-ptpn11-p-tyr-542-mab-2e2-262202413221510.jpg "Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Immunoprecipitating SHP-2/PTPN11 [p Tyr542] in Hela whole cell lysate treated with Pervanadate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26397 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26397 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg). Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
![Western Blot: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not). All lanes: SHP-2/PTPN11 [p Tyr542] Antibody at 0.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68 KDa Observed band size: 68 KDa](http://images.novusbio.com/fullsize/nbp3-26397_rabbit-shp-2-ptpn11-p-tyr-542-mab-2e2-28220241140942.jpg)
![Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.](http://images.novusbio.com/fullsize/nbp3-26251_rabbit-wnk1-mab-26g7-262202415355728.jpg "Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.")
![Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.](http://images.novusbio.com/fullsize/nbp3-26251_rabbit-wnk1-mab-26g7-272202485632.jpg)
![Western Blot: PKN2 Antibody (11H3) [NBP3-26378] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate. All lanes: PKN2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 140 kDa Observed band size: 140 kDa](http://images.novusbio.com/fullsize/nbp3-26378_rabbit-pkn2-mab-11h3-282202411351623.jpg "Western Blot: PKN2 Antibody (11H3) [NBP3-26378] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate. All lanes: PKN2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 140 kDa Observed band size: 140 kDa")
![Flow Cytometry: PKN2 Antibody (11H3) [NBP3-26378] - Overlay Peak curve showing HepG2 cells stained with PKN2 Antibody (11H3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26378_rabbit-pkn2-mab-11h3-282202414183697.jpg)