| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48926-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26666-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03045-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98908-50ul | |||
![Western Blot: Renal Cell Carcinoma (gp200) Antibody [NBP2-48926] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251MG sp, Lane 4: Human plasma (igG/HSa depleted).](http://images.novusbio.com/fullsize/Renal-Cell-Carcinoma-gp200-Antibody-Western-Blot-NBP2-48926-img0002.jpg "Western Blot: Renal Cell Carcinoma (gp200) Antibody [NBP2-48926] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251MG sp, Lane 4: Human plasma (igG/HSa depleted).")
![Immunohistochemistry: Renal Cell Carcinoma (gp200) Antibody [NBP2-48926] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Renal-Cell-Carcinoma-gp200-Antibody-Immunohistochemistry-NBP2-48926-img0001.jpg)
![Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-262202414471566.jpg "Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: CDK2 Antibody (6A5) [NBP3-26666] - Positive Western Blot detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate. All lanes: CDK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 31 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-282202411351622.jpg)
![Immunohistochemistry:[NBP3-30203]-Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1: 1,000. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30203_rabbit-yb1-pab-2312025128882.jpg "Immunohistochemistry:[NBP3-30203]-Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1: 1,000. Detection: DAB")
![Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-912202414154382.jpg "Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-822025209873.jpg)
![Western Blot: CDK2 Antibody [NBP3-03045] - Analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/CDK2-Antibody-Knockout-Validated-NBP3-03045-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CDK2 Antibody [NBP3-03045] - Analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: CDK2 Antibody - Azide and BSA Free [CDK2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] CDK2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03045_rabbit-polyclonal-cdk2-antibody-522025163631.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (PD00-19) [NBP3-32826] - Western blot analysis of Alkaline Phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Alkaline Phosphatase/ALPP antibody (NBP3-32826) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32826) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32826_rabbit-alkaline-phosphatase-alpp-mab-pd00-19-2882024142264.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (PD00-19) [NBP3-32826] - Western blot analysis of Alkaline Phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Alkaline Phosphatase/ALPP antibody (NBP3-32826) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32826) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (PD00-19) [NBP3-32826] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Alkaline Phosphatase/ALPP antibody (NBP3-32826) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32826) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32826_rabbit-alkaline-phosphatase-alpp-mab-pd00-19-29820241420925.jpg)
![Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/YB1-Antibody-Western-Blot-NBP2-98908-img0003.jpg "Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa")
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP2-98908] - Immunofluorescence staining of YB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98908 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98908_rabbit-polyclonal-yb1-antibody-235202514331553.png)
![Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk (C-terminal region) NBP3-23251 (lanes 1 & 2) and Crk (Tyr-221) phospho-specific NBP3-23252 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23252_rabbit-crk-p-tyr221-pab-19920231252363.jpg "Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk (C-terminal region) NBP3-23251 (lanes 1 & 2) and Crk (Tyr-221) phospho-specific NBP3-23252 (lanes 3 & 4).")
![Western Blot: Mouse Crk mAb (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).](http://images.novusbio.com/fullsize/antibody/nbp3-23251_mouse-crk-mab-m332-western-blot-308202312112..jpg "Western Blot: Mouse Crk mAb (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).")
![Western Blot: Crk Antibody (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23251_mouse-crk-mab-m332-19920231856959.jpg)