| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95102-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22663-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55954-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21134 | |||
![Western Blot: Mre11 Antibody [NBP2-95102] - Western blot analysis of extracts of various cell lines, using Mre11 antibody (NBP2-95102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Mre11 Antibody-Western Blot-NBP2-95102-img0002.jpg "Western Blot: Mre11 Antibody [NBP2-95102] - Western blot analysis of extracts of various cell lines, using Mre11 antibody (NBP2-95102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: Mre11 Antibody [NBP2-95102] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Mre11 antibody (NBP2-95102). Western blot was performed from the immunoprecipitate using Mre11 antibody (NBP2-95102) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunoprecipitation-NBP2-95102-img0001.jpg)
![Western Blot: Mre11 Antibody (8Q2B3) [NBP3-16359] - Analysis of extracts of various cell lines, using Mre11 Rabbit mAb (NBP3-16359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Mre11-Antibody-8Q2B3-Western-Blot-NBP3-16359-img0001.jpg "Western Blot: Mre11 Antibody (8Q2B3) [NBP3-16359] - Analysis of extracts of various cell lines, using Mre11 Rabbit mAb (NBP3-16359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: Mre11 Antibody (8Q2B3) [NBP3-16359] - Chromatin immunoprecipitation analysis of extracts of LNCaP cells, using Mre11 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16359_rabbit-mre11-mab-8q2b3-5220251242538.jpg)
![Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Thr2609]-Antibody-Western-Blot-NB600-897-img0005.jpg "Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.")
![Western Blot results of Rabbit DNA-PKcs [p Thr2609] Antibody. Lane 1: Molecular Weight Ladder. Lane 2: DNA Pkc phosphor control. Lane 3: DNA Pkc control. Lane 4: BSA. Load: 10ug. Primary Antibody: Rabbit Anti-DNA Pkcs pT609 at 1ug/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP](http://images.novusbio.com/fullsize/nb600-897_rabbit-polyclonal-dna-pkcs-p-thr2609-antibody-25520231328193.jpg)
![Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Western-Blot-NBP3-16355-img0001.jpg "Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Nbs1 Antibody (4G4R1) [NBP3-16355] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Nbs1 antibody (NBP3-16355). Western blot was performed from the immunoprecipitate using Nbs1 antibody (NBP3-16355) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Immunoprecipitation-NBP3-16355-img0002.jpg)
![Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32890_rabbit-rad50-mab-je60-04-288202412461633.jpg "Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: DNA-PKcs Antibody [NB100-660] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-660. DNA-PKcs was also IPed using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-658 and NB100-659.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-660-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-660] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-660. DNA-PKcs was also IPed using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-658 and NB100-659.")
![Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.](http://images.novusbio.com/fullsize/nbp3-22663_rabbit-nbs1-p-ser-343-mab-sr2302-57202317215135.jpg "Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.")
![SDS-Page: Recombinant Human, Nbs1 Protein [H00004683-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue, using Recombinant Human, Nbs1 Protein [H00004683-Q01].](http://images.novusbio.com/fullsize/qc_test-H00004683-Q01-1.jpg "SDS-Page: Recombinant Human, Nbs1 Protein [H00004683-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue, using Recombinant Human, Nbs1 Protein [H00004683-Q01].")
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP2-55954] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55954-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP2-55954] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.")
