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![Western Blot: PSD-95 Antibody (0M7N4) [NBP3-15251] - Western blot analysis of extracts of various cell lines, using PSD-95 Rabbit mAb (NBP3-15251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PSD-95-Antibody-0M7N4-Western-Blot-NBP3-15251-img0001.jpg "Western Blot: PSD-95 Antibody (0M7N4) [NBP3-15251] - Western blot analysis of extracts of various cell lines, using PSD-95 Rabbit mAb (NBP3-15251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (0M7N4) [PSD-95] - Confocal imaging of paraffin-embedded Mouse retina tissue using PSD-95 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15251_rabbit-psd-95-mab-0m7n4-52202517184594.jpg)
![Western Blot: PSD-95 Antibody (0M7N4) [PSD-95] - Western blot analysis of various lysates using PSD-95 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15251_rabbit-psd-95-mab-0m7n4-52202517192925.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: GABA-A R alpha 6 Antibody [NB300-150]](http://images.novusbio.com/fullsize/GABA-A-R-alpha-6-Antibody-Western-Blot-NB300-150-img0001.jpg "Western Blot: GABA-A R alpha 6 Antibody [NB300-150]")
![Western Blot: nNOS Antibody [NBP1-97555] - Analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-97555-img0003.jpg "Western Blot: nNOS Antibody [NBP1-97555] - Analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.")
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-97555] - Human brain, cortex tissue. Dilution at 20ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-97555-img0002.jpg)
![Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons](http://images.novusbio.com/fullsize/mu-Opioid-R-OPRM1-Antibody-Immunohistochemistry-Paraffin-NLS234-img0001.jpg "Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons")
![Immunohistochemistry-Paraffin: mGluR5 Antibody - BSA Free [NBP1-02331] - Staining of human colon, myenteric plexus. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/mGluR5-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02331-img0002.jpg "Immunohistochemistry-Paraffin: mGluR5 Antibody - BSA Free [NBP1-02331] - Staining of human colon, myenteric plexus. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6 ug/ml.")
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1779] - Analysis of anti-CRF1 antibody with rat hypothalalmus at 21 ug/ml.](http://images.novusbio.com/fullsize/CRHR1-CRF1-Antibody-Immunohistochemistry-Paraffin-NLS1779-img0004.jpg "Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1779] - Analysis of anti-CRF1 antibody with rat hypothalalmus at 21 ug/ml.")
![Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1779] - Analysis of anti-CRF1 antibody with human brain, neurons and glia at 21 ug/ml.](http://images.novusbio.com/fullsize/CRHR1-CRF1-Antibody-Immunohistochemistry-Paraffin-NLS1779-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT1F Antibody [NLS3343] - Analysis of anti-HTR1F / 5-HT1F antibody with human brain, cortex, neurons at dilution 2-3 ug/ml.](http://images.novusbio.com/fullsize/5-HT1F-Antibody-Immunohistochemistry-Paraffin-NLS3343-img0002.jpg "Immunohistochemistry-Paraffin: 5-HT1F Antibody [NLS3343] - Analysis of anti-HTR1F / 5-HT1F antibody with human brain, cortex, neurons at dilution 2-3 ug/ml.")
![Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-278202415212436.jpg "Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-298202414333648.jpg)