| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59326-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37281-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61775-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59700-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Western-Blot-NBP1-48319-img0004.jpg "Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Immunocytochemistry-Immunofluorescence-NBP1-48319-img0003.jpg)
![Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Western-Blot-NBP2-59326-img0007.jpg "Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0004.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0003.jpg "Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunocytochemistry-Immunofluorescence-NBP2-37281-img0004.jpg)
![Immunohistochemistry: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunohistochemistry-NBP2-37281-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0002.jpg)
![ELISA: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-ELISA-NBP2-37281-img0001.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0004.jpg "Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry-Paraffin: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of rectum cancer tissues using GRIA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Immunohistochemistry-NBP2-61775-img0003.jpg)
![Flow Cytometry: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Flow-Cytometry-NBP2-61775-img0002.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0005.jpg)
![ELISA: GluR2 Antibody (7A7A3) [NBP2-61775] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-ELISA-NBP2-61775-img0001.jpg)
![Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Western-Blot-NBP2-59700-img0002.jpg "Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody, Clone S415-24 (NBP2-59700). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody (NBP2-59700) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-AR alpha 5 Alpha 5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Immunocytochemistry-Immunofluorescence-NBP2-59700-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Immunocytochemistry-Immunofluorescence-NBP2-59700-img0001.jpg)
![Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]](http://images.novusbio.com/fullsize/DARPP-32-[p-Thr34]-Antibody-Western-Blot-NB300-224-img0001.jpg "Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]")
![Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05166-img0002.jpg "Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.")
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (interneurons in the cortex) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0004.jpg)
![Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-NBP3-14426-img0005.jpg "Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of breast carcinoma")
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, glioblastoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0001.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, adenocarcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0002.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0003.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, neurons and glia, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0004.jpg)
![Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of colon carcinoma](http://images.novusbio.com/fullsize/NPY5R Antibody-Immunohistochemistry-NBP3-14426-img0006.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)