| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38425-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94257-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05384-20ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB100-2658AFP488 | |||
| Alexa Fluor Plus 555 | NB100-2658AFP555 | |||
| Alexa Fluor Plus 594 | NB100-2658AFP594 | |||
| Alexa Fluor Plus 647 | NB100-2658AFP647 | |||
| Alexa Fluor Plus 680 | NB100-2658AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2658-0.025mg | |||
![Western Blot: Orai1 Antibody [NBP3-38425] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38425_rabbit-orai1-pab-1012202414043.jpg "Western Blot: Orai1 Antibody [NBP3-38425] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Orai1 Antibody [NBP3-38425] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38425_rabbit-orai1-pab-1012202414047.jpg)
![Western Blot: Plakophilin 2 Antibody [NBP2-94257] - Analysis of extracts of various cell lines, using Plakophilin 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Western-Blot-NBP2-94257-img0001.jpg "Western Blot: Plakophilin 2 Antibody [NBP2-94257] - Analysis of extracts of various cell lines, using Plakophilin 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Western Blot: Cathepsin B Antibody (6I5D7) [NBP3-15614] - Western blot analysis of extracts of HepG2 cells, using Cathepsin B antibody (NBP3-15614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-6I5D7-Western-Blot-NBP3-15614-img0002.jpg "Western Blot: Cathepsin B Antibody (6I5D7) [NBP3-15614] - Western blot analysis of extracts of HepG2 cells, using Cathepsin B antibody (NBP3-15614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunohistochemistry-Paraffin: Cathepsin B Antibody (6I5D7) [NBP3-15614] - Immunohistochemistry of paraffin-embedded human lung cancer using Cathepsin B Rabbit mAb (NBP3-15614) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-6I5D7-Immunohistochemistry-Paraffin-NBP3-15614-img0001.jpg)
![Western Blot: beta-Catenin [p Ser45] Antibody [NBP3-05442] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-05442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser45]-Antibody-Western-Blot-NBP3-05442-img0001.jpg "Western Blot: beta-Catenin [p Ser45] Antibody [NBP3-05442] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-05442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MDM2-HDM2-[p-Ser166]-Antibody-Western-Blot-NBP3-05384-img0002.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 [p Ser166] Antibody - BSA Free [MDM2/HDM2] - Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using MDM2/HDM2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-05384_rabbit-polyclonal-mdm2-hdm2-p-ser166-antibody-5220251640233.jpg)
![Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Western-Blot-NBP3-15631-img0001.jpg "Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Elk-1 antibody (NBP3-15631). Western blot was performed from the immunoprecipitate using Elk-1 antibody (NBP3-15631) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Immunoprecipitation-NBP3-15631-img0002.jpg)
![Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.")
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of various cell lines, using beta-Catenin [p Ser552] antibody (NBP3-05441) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0001.jpg)
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-91220241512139.jpg "Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of various lysates using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-912202414123886.jpg)
![Western Blot: EphB1 Antibody (5F10A4) [NBP1-28887] - Analysis using EhpB1 mouse mAb against MDA-MB-468 (1), MDA-MB-453 (2), MCF-7 (3), T47D (4) and SKBR-3 (5) cell lysate.](http://images.novusbio.com/fullsize/EphB1-Antibody-5F10A4-Western-Blot-NBP1-28887-img0005.jpg "Western Blot: EphB1 Antibody (5F10A4) [NBP1-28887] - Analysis using EhpB1 mouse mAb against MDA-MB-468 (1), MDA-MB-453 (2), MCF-7 (3), T47D (4) and SKBR-3 (5) cell lysate.")
![Immunohistochemistry-Paraffin: EphB1 Antibody (5F10A4) [NBP1-28887] - Analysis of human lung cancer (left) and colon cancer (right) showing cytoplasmic localization with DAB staining using EphB1 mouse mAb.](http://images.novusbio.com/fullsize/EphB1-Antibody-5F10A4-Immunohistochemistry-Paraffin-NBP1-28887-img0004.jpg)
![Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Western-Blot-NB300-235-img0001.jpg "Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Thr202] Antibody [NB300-235] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Immunohistochemistry-NB300-235-img0002.jpg)
![Western Blot: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).](http://images.novusbio.com/fullsize/Elk-1-Antibody-3H6D12-Western-Blot-NBP1-47553-img0002.jpg "Western Blot: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).")
![Immunohistochemistry-Paraffin: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis of human brain tumor tissue, showing nuclear and cytoplasmic localization using ELK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Elk-1-Antibody-3H6D12-Immunohistochemistry-Paraffin-NBP1-47553-img0003.jpg)