| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99278-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81038-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11943-25ul | |||
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Anti-GRM8 / MGLUR8 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0005.jpg "Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Anti-GRM8 / MGLUR8 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with brain, neurons.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0002.jpg)
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with human brain, neurons and glia at 7 ug/ml](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0004.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIIA/CD16a Antibody [NBP2-99278] - Staining Fc gamma RIIIA/CD16a in human spleen with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-Immunohistochemistry-Paraffin-NBP2-99278-img0004.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIIA/CD16a Antibody [NBP2-99278] - Staining Fc gamma RIIIA/CD16a in human spleen with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: Fc gamma RIIIA/CD16a Antibody [NBP2-99278] - Staining Fc gamma RIIIA/CD16a in cynomolgus liver with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-Immunohistochemistry-Paraffin-NBP2-99278-img0001.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIIA/CD16a Antibody [NBP2-99278] - Staining Fc gamma RIIIA/CD16a in cynomolgus spleen with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-Immunohistochemistry-Paraffin-NBP2-99278-img0002.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIIA/CD16a Antibody [NBP2-99278] - Staining Fc gamma RIIIA/CD16a in human liver with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-Immunohistochemistry-Paraffin-NBP2-99278-img0003.jpg)
![Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-278202415295520.jpg "Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRCP Antibody (JE65-42) [NBP3-32218] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CRCP antibody (NBP3-32218) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32218) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-298202414161851.jpg)
![Immunocytochemistry/ Immunofluorescence: CRCP Antibody (JE65-42) [NBP3-32218] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRCP with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-2920241033408.jpg)
![Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Western-Blot-NBP2-81078-img0002.jpg "Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.")
![Immunocytochemistry /Immunofluorescence: PrPSc Antibody (3F4) - Azide and BSA Free [NBP2-81078] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81078 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81078, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81078_mouse-monoclonal-prpsc-antibody-3f4-74202522265212.png)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-83-14-Flow-Cytometry-NBP2-81038-img0001.jpg "Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Western-Blot-NBP3-11943-img0001.jpg "Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.")
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (1G10) [NBP3-11943] - Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of A2BP1 (cat. NBP3-11943, 1:500, green).](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Immunocytochemistry-Immunofluorescence-NBP3-11943-img0002.jpg)
![Western Blot: FOG2 Antibody [NBP1-32895] - A. 50 ug rat brain lysate/extract 5 % SDS-PAGE FOG2/ZFPM2 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/FOG2-Antibody-Western-Blot-NBP1-32895-img0005.jpg "Western Blot: FOG2 Antibody [NBP1-32895] - A. 50 ug rat brain lysate/extract 5 % SDS-PAGE FOG2/ZFPM2 antibody dilution: 1:1000")
![Western Blot: FOG2 Antibody [NBP1-32895] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/FOG2-Antibody-Western-Blot-NBP1-32895-img0003.jpg)
![Western Blot: FOG2 Antibody [NBP1-32895] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FOG2-Antibody-Western-Blot-NBP1-32895-img0004.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0003.jpg)
![Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-NB100-77315-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.")
![Western Blot: S100 alpha 2 Antibody [NBP2-20247] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/S100-alpha-2-Antibody-Western-Blot-NBP2-20247-img0001.jpg "Western Blot: S100 alpha 2 Antibody [NBP2-20247] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: S100A2 Antibody [NBP2-20247] - Human normal kidney. S100A2 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/S100A2-Antibody-Immunohistochemistry-Paraffin-NBP2-20247-img0003.jpg)
![S100A2 antibody [N1C3] detects S100A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: S100A2 protein stained by S100A2 antibody [N1C3] (NBP2-20247) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-20247_rabbit-polyclonal-s100a2-antibody-immunocytochemistry-immunofluorescence-2611202518351711.jpg)
![Western Blot: CaMKIV Antibody [NBP1-78002] - Shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-Western-Blot-NBP1-78002-img0003.jpg "Western Blot: CaMKIV Antibody [NBP1-78002] - Shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Immunohistochemistry-Paraffin: CaMKIV Antibody [NBP1-78002] - Antibody was diluted 1:500 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-Immunohistochemistry-Paraffin-NBP1-78002-img0002.jpg)
