| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00194-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21978-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82003-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82000-0.025ml | |||
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0001.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251664755.png "Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in human skeletal muscle with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520252135113.png)
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in cynomolgus heart with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251433156.png)
![Western Blot: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32986) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32986_rabbit-synaptophysin-mab-je42-51-288202412493471.jpg "Western Blot: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32986) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32986) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32986_rabbit-synaptophysin-mab-je42-51-29820241420922.jpg)
![Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunohistochemistry-Paraffin-NB100-68244-img0002.jpg "Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRS2 Antibody [NB100-68244] - Detection of Human IRS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody NB100-68244 used for IP at 3 mcg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies NB100-68242 and NB100-68243, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunoprecipitation-NB100-68244-img0003.jpg)
![Western Blot: OMgp Antibody [NBP1-33647] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OMgp-Antibody-Western-Blot-NBP1-33647-img0003.jpg "Western Blot: OMgp Antibody [NBP1-33647] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Frozen: OMgp Antibody [NBP1-33647] - OMG antibody [C1C2] detects OMG protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: OMG antibody [C1C2] diluted at 1:1250. Blue: DAPI](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Frozen-NBP1-33647-img0004.jpg)
![Western Blot: APPL Antibody (S05-8D5) [NBP3-14998] - Western blot detection of APPL in Rat Brain, C6, 3T3, Hela cell lysates using APPL Rabbit mAb (1:1000 diluted). Predicted band size: 80kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/APPL-Antibody-S05-8D5-Western-Blot-NBP3-14998-img0001.jpg "Western Blot: APPL Antibody (S05-8D5) [NBP3-14998] - Western blot detection of APPL in Rat Brain, C6, 3T3, Hela cell lysates using APPL Rabbit mAb (1:1000 diluted). Predicted band size: 80kDa. Observed band size: 80kDa.")
![Immunohistochemistry-Paraffin: APPL Antibody (S05-8D5) [NBP3-14998] - Immunohistochemistry of APPL in paraffin-embedded Human lung cancer tissue using APPL Rabbit mAb at dilution 1/20](http://images.novusbio.com/fullsize/APPL-Antibody-S05-8D5-Immunohistochemistry-Paraffin-NBP3-14998-img0002.jpg)
![Western Blot: IRS2 Antibody (SR1279) [NBP3-21978] - Western blot analysis using NBP3-21978 in HEK293 cell treated with insulin.](http://images.novusbio.com/fullsize/nbp3-21978_rabbit-irs2-mab-sr1279-57202317215121.jpg "Western Blot: IRS2 Antibody (SR1279) [NBP3-21978] - Western blot analysis using NBP3-21978 in HEK293 cell treated with insulin.")
![Western Blot: IRS1 [p Ser636] Antibody [NB100-82003] - Analysis of extracts from 3T3 cells untreated or treated with Insulin using IRS-1 (Phospho-Ser636) Antibody](http://images.novusbio.com/fullsize/IRS1-[p-Ser636]-Antibody-Western-Blot-NB100-82003-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRS1 [p Ser636] Antibody [NB100-82003] - Analysis of extracts from 3T3 cells untreated or treated with Insulin using IRS-1 (Phospho-Ser636) Antibody")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IRS1 [p Ser636] Antibody [NB100-82003] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82003(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82003_rabbit-polyclonal-irs1-p-ser636-antibody-103202523464540.jpg)
![Western Blot: IRS1 [p Ser639] Antibody [NB100-82000] - Analysis of extracts from 293 cells untreated or treated with serum using IRS-1 (Phospho-Ser639) antibody](http://images.novusbio.com/fullsize/IRS1-[p-Ser639]-Antibody-Western-Blot-NB100-82000-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRS1 [p Ser639] Antibody [NB100-82000] - Analysis of extracts from 293 cells untreated or treated with serum using IRS-1 (Phospho-Ser639) antibody")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IRS1 [p Ser639] Antibody [NB100-82000] - Analysis of breast carcinoma tissue, using NB100-82000(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82000_rabbit-polyclonal-irs1-p-ser639-antibody-10320252332102.jpg)
![Western Blot: CRYGS Antibody [NBP3-12221] - WB of NBP3-12221 ith 1:750 antibody ailution in DiluObuffer. Apparent MW is 21 kDa.](http://images.novusbio.com/fullsize/CRYGS-Antibody-Western-Blot-NBP3-12221-img0001.jpg "Western Blot: CRYGS Antibody [NBP3-12221] - WB of NBP3-12221 ith 1:750 antibody ailution in DiluObuffer. Apparent MW is 21 kDa.")
![Western Blot: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Western-Blot-NBP3-08773-img0003.jpg "Western Blot: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08773-img0002.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08773-img0001.jpg)