| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89254-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90051-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06199-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98717-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97410-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90701-50ul | |||
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (027) [NBP2-89254] - Staining of Human BACE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/BACE-1-Antibody-027-Immunocytochemistry-Immunofluorescence-NBP2-89254-img0001.jpg "Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (027) [NBP2-89254] - Staining of Human BACE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: BACE-1 Antibody (027) [NBP2-89254] - Analysis of Human BACE1 expression on Jurkat cells. The cells were treated stained with purified anti-Human BACE1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/BACE-1-Antibody-027-Flow-Cytometry-NBP2-89254-img0002.jpg)
![Western Blot: BACE-1 Antibody (JE30-40) [NBP3-32066] - Western blot analysis of BACE-1 on different lysates with Rabbit anti-BACE-1 antibody (NBP3-32066) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Mouse cerebellum tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32066) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32066_rabbit-bace-1-mab-je30-40-278202416331246.jpg "Western Blot: BACE-1 Antibody (JE30-40) [NBP3-32066] - Western blot analysis of BACE-1 on different lysates with Rabbit anti-BACE-1 antibody (NBP3-32066) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Mouse cerebellum tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32066) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - Lane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:18 kDa](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-017-Western-Blot-NBP2-90051-img0001.jpg "Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - Lane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:18 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - NBP2-90051 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: alpha-Synuclein knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 16 kDa](http://images.novusbio.com/fullsize/nbp2-90051_rabbit-monoclonal-alpha-synuclein-antibody-017-136202422345017.png)
![Western Blot: Numb Antibody [NBP3-30927] - Western blot using anti-Numb antibody (Cat. No. NBP3-30927) on human brain lysate (14ug/lane). Antibody was used with (lane 1) and without (lane 2) blocking peptide at 10ug/ml.](http://images.novusbio.com/fullsize/nbp3-30927_rabbit-numb-pab-81120242059219.jpg "Western Blot: Numb Antibody [NBP3-30927] - Western blot using anti-Numb antibody (Cat. No. NBP3-30927) on human brain lysate (14ug/lane). Antibody was used with (lane 1) and without (lane 2) blocking peptide at 10ug/ml.")
![Immunohistochemistry-Paraffin: Hippocalcin Antibody [NBP3-06199] - Immunochemical staining of human HPCA in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Hippocalcin-Antibody-Immunohistochemistry-Paraffin-NBP3-06199-img0002.jpg "Immunohistochemistry-Paraffin: Hippocalcin Antibody [NBP3-06199] - Immunochemical staining of human HPCA in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Hippocalcin Antibody [NBP3-06199] - Immunochemical staining of human HPCA in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Hippocalcin-Antibody-Immunohistochemistry-Paraffin-NBP3-06199-img0001.jpg)
![Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Western-Blot-NBP2-98717-img0002.jpg "Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Numb Antibody [NBP2-98717] - Numb was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Numb rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Numb rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Immunoprecipitation-NBP2-98717-img0001.jpg)
![Western Blot: CLCN6 Antibody [NBP2-84690] - Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Gene Name: CLCN6. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science.](http://images.novusbio.com/fullsize/CLCN6-Antibody-Western-Blot-NBP2-84690-img0002.jpg "Western Blot: CLCN6 Antibody [NBP2-84690] - Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Gene Name: CLCN6. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science.")
![Western Blot: CLCN6 Antibody [NBP2-84690] - WB Suggested Anti-CLCN6 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/CLCN6-Antibody-Western-Blot-NBP2-84690-img0001.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP2-97410] - Immunochemical staining of human PPT1 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97410-img0002.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody [NBP2-97410] - Immunochemical staining of human PPT1 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP2-97410] - Immunochemical staining of human PPT1 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97410-img0001.jpg)
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Western-Blot-NBP2-90701-img0003.jpg "Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
![Flow Cytometry: Syndecan-4 Antibody (112) [NBP2-90701] - Analysis of Mouse SDC4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SDC4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Flow-Cytometry-NBP2-90701-img0002.jpg)
![Western Blot: Kv1.5 Antibody [NBP3-30822] - Western blot analysis using Kv1.5 antibody on rat brain lysate.](http://images.novusbio.com/fullsize/nbp3-30822_rabbit-kv1-5-pab-811202420594211.jpg "Western Blot: Kv1.5 Antibody [NBP3-30822] - Western blot analysis using Kv1.5 antibody on rat brain lysate.")
![Western Blot: Nav1.6 Antibody [NBP2-84176] - WB Suggested Anti-SCN8A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/Nav1.6-Antibody-Western-Blot-NBP2-84176-img0001.jpg "Western Blot: Nav1.6 Antibody [NBP2-84176] - WB Suggested Anti-SCN8A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle")
![Western Blot: Nav1.6 Antibody [NBP2-84176] - Host: Rabbit. Target Name: SCN8A. Sample Type: NCI-H226. Antibody Dilution: 1.0ug/mlSCN8A is supported by BioGPS gene expression data to be expressed in NCIH226](http://images.novusbio.com/fullsize/Nav1.6-Antibody-Western-Blot-NBP2-84176-img0002.jpg)