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![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-89294-img0003.jpg "Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294] - Staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to myenteric nerve plexus.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunohistochemistry-Paraffin-NBP2-89294-img0001.jpg)
![Flow Cytometry: L1CAM Antibody (014) [NBP2-89294] - Analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Flow-Cytometry-NBP2-89294-img0002.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-1992023185690.jpg "Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-119202316331615.jpg)
![Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856930.jpg "Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856954.jpg)
![Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-199202312523619.jpg "Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody [NBP3-23182] - Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NBP3-23182) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide in the presence of the anti-NRP-1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-19920231856956.jpg)
![Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Western-Blot-NBP2-79824-img0001.jpg "Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0002.jpg)
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0003.jpg)
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-42167] - DAB staining on IHC-P; Samples: Rat Spleen Tissue; Primary Ab: 10ug/ml NBP3-42167; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42167_rabbit-mt-nd5-pab-612202421373043.jpg "Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-42167] - DAB staining on IHC-P; Samples: Rat Spleen Tissue; Primary Ab: 10ug/ml NBP3-42167; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![Western Blot: MT-ND5 Antibody [NBP3-42167] - Sample: Lane1: Rat Serum; Lane2: Rat Heart lysate; Lane3: Rat Cerebrum lysate Primary Ab: 5ug/ml NBP3-42167; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42167_rabbit-mt-nd5-pab-612202421121373.jpg)
![Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-42167] - DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10ug/ml NBP3-42167; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42167_rabbit-mt-nd5-pab-61220242151854.jpg)
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-262202415355720.jpg "Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-27220248471912.jpg)
![Western Blot: ATPB Antibody (5F10) [NBP3-26490] - Positive Western Blot detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue. All lanes: ATPB Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-282202411363015.jpg)