| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37734-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37288-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97928-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06496-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
![Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Western-Blot-NBP2-37734-img0002.jpg "Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)")
![Flow Cytometry: MAP2 Antibody (5B7) [NBP2-37734] - Flow cytometric analysis of HeLa cells using MAP2 Antibody (5B7) (green) and negative control (red).](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Flow-Cytometry-NBP2-37734-img0003.jpg)
![ELISA: MAP2 Antibody (5B7) [NBP2-37734] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-ELISA-NBP2-37734-img0001.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0003.jpg "Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Flow cytometric analysis of Hela cells using PPP1R1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Flow-Cytometry-NBP2-37288-img0004.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0002.jpg)
![ELISA: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-ELISA-NBP2-37288-img0001.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-97928-img0007.jpg "Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.")
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Immunohistochemistry-NBP1-97928-img0008.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Western Blot: GliS2 Antibody [NBP2-84979] - Host: Rabbit. Target Name: GLIS2. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/GliS2-Antibody-Western-Blot-NBP2-84979-img0003.jpg "Western Blot: GliS2 Antibody [NBP2-84979] - Host: Rabbit. Target Name: GLIS2. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: GliS2 Antibody [NBP2-84979] - Sample Type: Human Bile DuctPrimary. Dilution: 1:500](http://images.novusbio.com/fullsize/GliS2-Antibody-Immunohistochemistry-NBP2-84979-img0001.jpg)
![Western Blot: GliS2 Antibody [NBP2-84979] - Host: Rabbit. Target Name: GLIS2. Sample Tissue: Human Ovary Tumor. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/GliS2-Antibody-Western-Blot-NBP2-84979-img0002.jpg)
![Western Blot: GliS2 Antibody [NBP2-84979] - Host: Rabbit. Target: GLIS2. Positive control (+): Human Ovary Tumor (T-OV). Negative control (-): Human Liver Tumor (T-LI). Antibody concentration: 3ug/ml](http://images.novusbio.com/fullsize/GliS2-Antibody-Western-Blot-NBP2-84979-img0004.jpg)
![Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-278202416331210.jpg "Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody (JE31-53) [NBP3-32224] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRMP2 with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202410484827.jpg)
![Flow Cytometry: CRMP2 Antibody (JE31-53) [NBP3-32224] - Flow cytometric analysis of SH-SY5Y cells labeling CRMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32224, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202416372179.jpg)
![Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-278202416285223.jpg "Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Immunocytochemistry analysis of A549 cells labeling Galactosylceramidase/GALC with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-29202410234291.jpg)
![Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Western-Blot-NBP3-06496-img0001.jpg "Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa")
![Immunohistochemistry-Paraffin: CEND1 Antibody [NBP3-06496] - Staining of human CEND1 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunohistochemistry-Paraffin-NBP3-06496-img0002.jpg)
![Immunoprecipitation: CEND1 Antibody [NBP3-06496] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-CEND1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CEND1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunoprecipitation-NBP3-06496-img0003.jpg)
![Western Blot: CRMP4 Antibody [NBP2-87213] - WB Suggested Anti-DPYSL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T](http://images.novusbio.com/fullsize/CRMP4-Antibody-Western-Blot-NBP2-87213-img0003.jpg "Western Blot: CRMP4 Antibody [NBP2-87213] - WB Suggested Anti-DPYSL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T")
![Immunohistochemistry: CRMP4 Antibody [NBP2-87213] - Immunohistochemistry with Small intestine, submucosal plexus tissue. Antibody concentration: 10ug/ml](http://images.novusbio.com/fullsize/CRMP4-Antibody-Immunohistochemistry-NBP2-87213-img0001.jpg)
![Immunoprecipitation: CRMP4 Antibody [NBP2-87213] - DRYSL3 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with NBP2-87213 with 1:200 dilution. Western blot was performed using NBP2-87213 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: DRYSL3 IP with NBP2-87213](http://images.novusbio.com/fullsize/CRMP4-Antibody-Immunoprecipitation-NBP2-87213-img0004.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-288202412461662.jpg "Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg)
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)