| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-158SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81038-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11943-25ul | |||
![Western Blot: MAS1 Antibody [NBP1-60091] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/MAS1-Antibody-Western-Blot-NBP1-60091-img0003.jpg "Western Blot: MAS1 Antibody [NBP1-60091] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: MAS1 Antibody [NBP1-60091] - Human Heart Tissue, antibody concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/MAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-60091-img0002.jpg)
![Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB100-1541-img0002.jpg "Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Flow-Cytometry-NB100-1541-img0001.jpg)
![Western Blot: APP Antibody [NB300-308]](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NB300-308-img0001.jpg "Western Blot: APP Antibody [NB300-308]")
![Immunocytochemistry/Immunofluorescence: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) APP. Overlay images indicate the possible co-localization of VDR/APP on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-308-img0002.jpg)
![Western Blot: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation & immunofluorescence labeling of target proteins (100x): A) ADAM10; B) APP; C) BACE1; D) Nicastrin; E) Presenilin 1; F) Presenilin 2. Overlay images indicate the possible co-localization of VDR/ADAM10 (A) or VDR/APP (B) or VDR/Nicastrin (D) or limited co-localization of VDR/ Presenilin 1 (E) on the neuronal plasma membrane. Overlay images do not indicate co-localization for VDR/BACE1 (C) or VDR/Presenilin 2 (F) on the neuronal plasma membrane. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-308_rabbit-polyclonal-app-antibody-31020241533495.jpg)
![Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Western-Blot-NB300-158-img0002.jpg "Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.")
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-83-14-Flow-Cytometry-NBP2-81038-img0001.jpg "Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Western-Blot-NBP2-81078-img0002.jpg "Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.")
![Immunocytochemistry /Immunofluorescence: PrPSc Antibody (3F4) - Azide and BSA Free [NBP2-81078] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81078 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81078, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81078_mouse-monoclonal-prpsc-antibody-3f4-74202522265212.png)
![Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Western-Blot-NBP3-11943-img0001.jpg "Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.")
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (1G10) [NBP3-11943] - Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of A2BP1 (cat. NBP3-11943, 1:500, green).](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Immunocytochemistry-Immunofluorescence-NBP3-11943-img0002.jpg)
![Western Blot: Syntaxin 3 Antibody [NBP2-15131] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Western-Blot-NBP2-15131-img0003.jpg "Western Blot: Syntaxin 3 Antibody [NBP2-15131] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Syntaxin 3 Antibody [NBP2-15131] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Western-Blot-NBP2-15131-img0001.jpg)
![Syntaxin 3 antibody [N1C2] detects Syntaxin 3 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Syntaxin 3 protein stained by Syntaxin 3 antibody [N1C2] (NBP2-15131) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15131_rabbit-polyclonal-syntaxin-3-antibody-immunocytochemistry-immunofluorescence-2611202519366.jpg)
![Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-NB100-77315-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.")