| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05527-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83104-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48998-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05526-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37937-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82907-25ul | |||
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05527] - used at a dilution of 1:1,000, in green. [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex, [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human Adenylate Cyclase 3 from the appropriate cDNA. This antibody detects variably glycosylated forms of Adenylate Cyclase 3 protein with apparent molecular weights from ~120kDa and higher. The strong band at about 130kDa demonstrates overexpression of the Adenylate Cyclase 3 protein, and those over 250kDA double band presumably corresponds to heavily glycosolated or aggregated forms of Adenylate Cyclase 3. The same blot was simultaneously probed with mouse monoclonal antibody to GAPDH at a at a dilution of of 1:5,000, in red, which reveals the single GAPDH band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-05527-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05527] - used at a dilution of 1:1,000, in green. [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex, [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human Adenylate Cyclase 3 from the appropriate cDNA. This antibody detects variably glycosylated forms of Adenylate Cyclase 3 protein with apparent molecular weights from ~120kDa and higher. The strong band at about 130kDa demonstrates overexpression of the Adenylate Cyclase 3 protein, and those over 250kDA double band presumably corresponds to heavily glycosolated or aggregated forms of Adenylate Cyclase 3. The same blot was simultaneously probed with mouse monoclonal antibody to GAPDH at a at a dilution of of 1:5,000, in red, which reveals the single GAPDH band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05527] - Immunofluorescence analysis of rat cortex section stained with Adenylate Cyclase 3 Antibody at a at a dilution of of 1:10,000, in red and costained with mouse monoclonal antibody to the myelin and oligodendrocyte marker CNP at a at a dilution of of 1:1,000 in green. The blue is Hoechst staining of nuclear DNA. The Adenylate Cyclase 3 Antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05527-img0001.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0006.jpg "Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0005.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0007.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-48998] - Staining of human cell line ASC TERT1 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48998-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0004.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0005.jpg)
![Western Blot: COX6A1 Antibody [NBP2-46865] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG. Lane 4: Human plasma Lane 5: Human Liver tissue. Lane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/COX6A1-Antibody-Western-Blot-NBP2-46865-img0002.jpg "Western Blot: COX6A1 Antibody [NBP2-46865] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG. Lane 4: Human plasma Lane 5: Human Liver tissue. Lane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: COX6A1 Antibody [NBP2-46865] - Staining of human cell line PC-3 shows localization to mitochondria.](http://images.novusbio.com/fullsize/COX6A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-46865-img0003.jpg)
![Immunohistochemistry-Paraffin: COX6A1 Antibody [NBP2-46865] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/COX6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-46865-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526] - Western blot analysis of HEK293 cell lysates using Adenylate Cyclase 3 Antibody, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with a vector encoding Myc-DDK tagged fill length human adenylate cyclase 3. The strong band at about 130kDa in transfected cells demonstrates overexpression of the human adenylate cyclase 3 protein, and bands over 250kDa presumably correspond to either heavily glycosolated form of adenylate cyclase 3 or multimeric forms of the protein. The same blot was simultaneously probed with rabbit polyclonal antibody to GAPDH dilution 1:20,000 in red, which reveals the single band at ~37kDa seen in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-05526-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526] - Western blot analysis of HEK293 cell lysates using Adenylate Cyclase 3 Antibody, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with a vector encoding Myc-DDK tagged fill length human adenylate cyclase 3. The strong band at about 130kDa in transfected cells demonstrates overexpression of the human adenylate cyclase 3 protein, and bands over 250kDa presumably correspond to either heavily glycosolated form of adenylate cyclase 3 or multimeric forms of the protein. The same blot was simultaneously probed with rabbit polyclonal antibody to GAPDH dilution 1:20,000 in red, which reveals the single band at ~37kDa seen in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05526] - Immunofluoresence of caudate/putamen region of rat brain stained with Adenylate Cyclase 3 Antibody in red, and chicken antibody to tyrosine hydroxylase, in green. The Blue is Hoechst stain revealing nuclei. The Adenylate Cyclase 3 Antibody reveals neuronal cilia while the tyrosine hydroxylase antibody reveals the axons of chatecholaminergic neurons.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05526-img0001.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-12369] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 129 KDa.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-12369-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-12369] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 129 KDa.")
![Immunohistochemistry-Paraffin: Adenylate Cyclase 3 Antibody [NBP3-12369] - Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12369-img0001.jpg)
![Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-3E2-E6-Western-Blot-H00006233-M01-img0002.jpg "Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.")
![Western Blot: CPEB3 Antibody [NBP1-56919] - Analysis of 721_B whole cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 0.12% .](http://images.novusbio.com/fullsize/CPEB3-Antibody-Western-Blot-NBP1-56919-img0004.jpg "Western Blot: CPEB3 Antibody [NBP1-56919] - Analysis of 721_B whole cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 0.12% .")
![Western Blot: CPEB3 Antibody [NBP1-56919] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPEB3-Antibody-Western-Blot-NBP1-56919-img0002.jpg)
![Immunohistochemistry: IP3R1 Antibody [NBP3-37937] - Perform microwave antigen retrievalwith 10 mM citrate buffer pH 6.0before commencing with IF stainingprotocol.Immunofluorescenceanalysis of paraffin-embedded RatBrain using IP3R Rabbit pAb(A21471) at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG(H+L) (AS007) at 1:500 dilution. Blue:DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-9122024159564.jpg "Immunohistochemistry: IP3R1 Antibody [NBP3-37937] - Perform microwave antigen retrievalwith 10 mM citrate buffer pH 6.0before commencing with IF stainingprotocol.Immunofluorescenceanalysis of paraffin-embedded RatBrain using IP3R Rabbit pAb(A21471) at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG(H+L) (AS007) at 1:500 dilution. Blue:DAPI for nuclear staining.")
![Immunoprecipitation: IP3R1 Antibody [NBP3-37937] - Immunoprecipitation of IP3R1 from 500 ug extracts of SH-SY5Y cells was performed using 2 ug of IP3R1 Rabbit pAb was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western Blot analysis of immunoprecipitates was conducted using IP3R1 Rabbit pAb at a dilution of 1:2500.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-31120251704798.jpg)
![Western Blot: IP3R1 Antibody [NBP3-37937] - Western Blot analysis of various lysates using IP3R1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): A-431.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-311202517310.jpg)
![Western Blot: COX6A1 Antibody (OTI4G2) - Azide and BSA Free [NBP2-70427] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COX6A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COX6A1.](http://images.novusbio.com/fullsize/COX6A1-Antibody-OTI4G2-Azide-and-BSA-Free-Western-Blot-NBP2-70427-img0001.jpg "Western Blot: COX6A1 Antibody (OTI4G2) - Azide and BSA Free [NBP2-70427] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COX6A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COX6A1.")
![Immunocytochemistry/Immunofluorescence: COX6A1 Antibody (OTI4G2) - Azide and BSA Free [NBP2-70427] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY COX6A1.](http://images.novusbio.com/fullsize/COX6A1-Antibody-OTI4G2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70427-img0003.jpg)
![Flow Cytometry: COX6A1 Antibody (OTI4G2) - Azide and BSA Free [NBP2-70427] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-COX6A1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/COX6A1-Antibody-OTI4G2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70427-img0002.jpg)
![Western Blot: Begain Antibody [NBP1-82907] - Analysis in control (vector only transfected HEK293T lysate) and BEGAIN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Begain-Antibody-Western-Blot-NBP1-82907-img0007.jpg "Western Blot: Begain Antibody [NBP1-82907] - Analysis in control (vector only transfected HEK293T lysate) and BEGAIN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Begain Antibody [NBP1-82907] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Begain-Antibody-Immunohistochemistry-Paraffin-NBP1-82907-img0006.jpg)