| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14227 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08909 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77262-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41286-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61710-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61768-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41364-0.025mg | |||
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-7081R-Immunohistochemistry-Paraffin-NBP3-13834-img0002.jpg "Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-7081R-Immunohistochemistry-Paraffin-NBP3-13834-img0001.jpg)
![Western Blot: CD45RB Antibody (rPTPRC/1132) [NBP3-07166] - Western Blot Analysis of human spleen cell lysate using CD45RB Recombinant Mouse Monoclonal Antibody (rPTPRC/1132).](http://images.novusbio.com/fullsize/CD45RB-Antibody-rPTPRC-1132-Western-Blot-NBP3-07166-img0002.jpg "Western Blot: CD45RB Antibody (rPTPRC/1132) [NBP3-07166] - Western Blot Analysis of human spleen cell lysate using CD45RB Recombinant Mouse Monoclonal Antibody (rPTPRC/1132).")
![Immunohistochemistry-Paraffin: CD45RB Antibody (rPTPRC/1132) [NBP3-07166] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45RB Recombinant Mouse Monoclonal Antibody (rPTPRC/1132).](http://images.novusbio.com/fullsize/CD45RB-Antibody-rPTPRC-1132-Immunohistochemistry-Paraffin-NBP3-07166-img0001.jpg)
![Western Blot: TRPC3 Antibody [NBP1-77262] - Human brain tissue lysate. (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Western-Blot-NBP1-77262-img0005.jpg "Western Blot: TRPC3 Antibody [NBP1-77262] - Human brain tissue lysate. (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry-Paraffin: TRPC3 Antibody [NBP1-77262] - Mouse heart tissue with TRPC3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Immunohistochemistry-Paraffin-NBP1-77262-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPC3 Antibody - BSA Free [NBP1-77262] - Immunofluorescence of TRPC3 in Mouse Heart cells with TRPC3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77262_rabbit-polyclonal-trpc3-antibody-2711202419541566.jpg)
![Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP2-41286-img0004.jpg "Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.")
![Immunohistochemistry: HDAC6 Antibody - BSA Free [NBP2-41286] - Immunohistochemistry of HDAC6 in human testis tissue with HDAC6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41286_rabbit-polyclonal-hdac6-antibody-2711202419392757.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody - BSA Free [NBP2-41286] - Immunofluorescence of HDAC6 in human testis tissue with HDAC6 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41286_rabbit-polyclonal-hdac6-antibody-2711202419241352.jpg)
![Western Blot: MAS1 Antibody [NBP1-60091] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/MAS1-Antibody-Western-Blot-NBP1-60091-img0003.jpg "Western Blot: MAS1 Antibody [NBP1-60091] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: MAS1 Antibody [NBP1-60091] - Human Heart Tissue, antibody concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/MAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-60091-img0002.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0004.jpg "Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis of MCF-7 cells using ADRB2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Flow-Cytometry-NBP2-61710-img0002.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against human ADRB2 (AA: 302-413) recombinant protein. (Expected MW is 38.5 kDa)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0003.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0005.jpg)
![ELISA: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-ELISA-NBP2-61710-img0001.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0004.jpg "Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0005.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0001.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0002.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Immunohistochemistry-NBP2-41364-img0005.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]")
![Sandwich ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364] - Analysis using the Biotin conjugate of NBP2-41364. The calibration curveusing NBP2-23595 as the biotinylated detection antibody and NBP2-41364 as the capture antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Sandwich-ELISA-NBP2-41364-img0004.jpg)
![Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-278202415295520.jpg "Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRCP Antibody (JE65-42) [NBP3-32218] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CRCP antibody (NBP3-32218) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32218) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-298202414161851.jpg)
![Immunocytochemistry/ Immunofluorescence: CRCP Antibody (JE65-42) [NBP3-32218] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRCP with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-2920241033408.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Western blot analysis of Dihydrolipoamide Dehydrogenase/DLD on different lysates with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HepG2 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32539) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-27820241521245.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Western blot analysis of Dihydrolipoamide Dehydrogenase/DLD on different lysates with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HepG2 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32539) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32539) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29820241492614.jpg)
![Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunocytochemistry analysis of HeLa cells labeling Dihydrolipoamide Dehydrogenase/DLD with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29202410234295.jpg)
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)