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![Western Blot: PAK1/2/3 [p Thr402] Antibody [NB100-723] - Rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in -Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/PAK1-2-3-[p-Thr402]-Antibody-Western-Blot-NB100-723-img0001.jpg "Western Blot: PAK1/2/3 [p Thr402] Antibody [NB100-723] - Rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in -Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Ki67/MKI67 Antibody (PD00-10) [NBP3-32453] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Ki67/MKI67 antibody (NBP3-32453) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32453) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32453_mouse-ki67-mki67-mab-pd00-10-298202414333629.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (PD00-10) [NBP3-32453] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Ki67/MKI67 antibody (NBP3-32453) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32453) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-20777-img0001.jpg "Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.")
![Western Blot: Kif4A Antibody (SR1195) [NBP3-22228] - Western blot analysis using NBP3-22228 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22228_rabbit-kif4a-mab-sr1195-5720231649426.jpg "Western Blot: Kif4A Antibody (SR1195) [NBP3-22228] - Western blot analysis using NBP3-22228 in HeLa cell lysate.")
![Western Blot: Tau Antibody (S04-2G8) [NBP3-20060] - Western blot detection of Tau in Rat Brain lysates using NBP3-20060 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S04-2G8-Western-Blot-NBP3-20060-img0001.jpg "Western Blot: Tau Antibody (S04-2G8) [NBP3-20060] - Western blot detection of Tau in Rat Brain lysates using NBP3-20060 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: alpha Adaptin Antibody [NBP3-13154] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Adaptin antibody (NBP3-13154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-Western-Blot-NBP3-13154-img0001.jpg "Western Blot: alpha Adaptin Antibody [NBP3-13154] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Adaptin antibody (NBP3-13154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45162_rabbit-tau-p-ser235-mab-hl3899-azide-and-bsa-free-western-blot-2942025155710..jpg "<b>Biological Strategies Validation. </b>Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CLN5 Antibody [NBP3-12971] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CLN5 antibody (NBP3-12971) diluted at 1:1000.](http://images.novusbio.com/fullsize/CLN5-Antibody-Western-Blot-NBP3-12971-img0001.jpg "Western Blot: CLN5 Antibody [NBP3-12971] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CLN5 antibody (NBP3-12971) diluted at 1:1000.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-06994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06994-img0001.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-06994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")