| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB2416-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44978 | |||
| Azide and BSA Free | NBP2-34605-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92692SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59632 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26333-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Western-Blot-NBP2-67617-img0006.jpg "Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate")
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunohistochemistry-Paraffin-NBP2-67617-img0005.jpg)
![Flow Cytometry: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Flow-Cytometry-NBP2-67617-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0003.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nNOS-Antibody-60718-Western-Blot-MAB2416-img0001.jpg "Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Western Blot: nNOS Antibody (60718) [MAB2416] - Effects of IFN alpha and IFN gamma treatment on nNOS expression levels in human metastatic melanoma A375 cells. Cells were treated with IFN alpha or IFN gamma for various timepoints and whole cell lysates were collected. Samples were subjected to Western blot analysis for nNOS. A control of the protein loading was performed by detecting actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35105915/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mab2416_mouse-monoclonal-nnos-antibody-60718-2552023153952.jpg)
![Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Western-Blot-NBP2-34308-img0004.jpg "Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).")
![Immunohistochemistry-Paraffin: p53 Antibody (DO-7) [NBP2-34308] - Formalin-fixed, paraffin-embedded human colon stained with p53 MAb (DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Immunohistochemistry-Paraffin-NBP2-34308-img0001.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0002.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0003.jpg)
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Western-Blot-NBP1-92692-img0005.jpg "Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.")
![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-92692 antibody reveals strong granular staining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - HeLa cells stained with NBP1-92692 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (blue, stained with DAPI). The SF3B4 (3A1) antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0003.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0005.jpg "Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).")
![Immunohistochemistry-Paraffin: p53 Antibody (TP53/1799R) [NBP2-59631] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withp53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R)](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Immunohistochemistry-Paraffin-NBP2-59631-img0002.jpg)
![Flow Cytometry: p53 Antibody (TP53/1799R) [NBP2-59631] - Flow Cytometric Analysis of HeLa cells using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Flow-Cytometry-NBP2-59631-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0003.jpg)
![Protein Array: p53 Antibody (TP53/1799R) [NBP2-59631] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Protein-Array-NBP2-59631-img0001.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg)
![Western Blot: Syntenin 1 Antibody (19C1) [NBP3-26333] - Positive Western Blot detected in: HT29 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate. All lanes: Syntenin 1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241147217.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (19C1) [NBP3-26333] - Staining of Hela with Syntenin 1 Antibody (19C1) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 521-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241249364.jpg)
![Flow Cytometry: Syntenin 1 Antibody (19C1) [NBP3-26333] - Overlay Peak curve showing HepG2 cells stained with Syntenin 1 Antibody (19C1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-282202414312716.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)