| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26492-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48192 | |||
| Azide and BSA Free | NBP3-11475 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22060-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21941-50ul | |||
![Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-2722024820398.jpg "Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.")
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Staining of MCF7 with SERCA2 ATPase Antibody (20D1) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-28220241247516.jpg)
![Flow Cytometry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Overlay Peak curve showing Hela cells stained with SERCA2 ATPase Antibody (20D1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-282202414312788.jpg)
![Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Western-Blot-NBP2-32258-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for IP at 6 ug per reaction. TRPC3 was also immunoprecipitated by rabbit anti-TRPC3 antibody BL16089. For blotting immunoprecipitated TRPC3, NBP2-32258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Immunoprecipitation-NBP2-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/784) [NBP2-44716] - Human Colon stained with CD56 Monoclonal Antibody (NCAM1/784)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-NCAM1-784-Immunohistochemistry-Paraffin-NBP2-44716-img0001.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/784) [NBP2-44716] - Human Colon stained with CD56 Monoclonal Antibody (NCAM1/784)")
![Western Blot: CEND1 Antibody (SR1342) [NBP3-22060] - Western blot analysis using NBP3-22060 in U-87 MG cell lysate.](http://images.novusbio.com/fullsize/nbp3-22060_rabbit-cend1-mab-sr1342-57202317161881.jpg "Western Blot: CEND1 Antibody (SR1342) [NBP3-22060] - Western blot analysis using NBP3-22060 in U-87 MG cell lysate.")
![Western Blot: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Western blot detection of EWSR1 in MCF7, 3T3, Hela cell lysates using EWSR1 Rabbit mAb (1:1000 diluted). Predicted band size: 69KDa. Observed band size: 85KDa.](http://images.novusbio.com/fullsize/EWSR1-Antibody-S09-3A7-Western-Blot-NBP3-14931-img0001.jpg "Western Blot: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Western blot detection of EWSR1 in MCF7, 3T3, Hela cell lysates using EWSR1 Rabbit mAb (1:1000 diluted). Predicted band size: 69KDa. Observed band size: 85KDa.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Immunofluorescence of EWSR1 (green) in Hela cells using NBP3-14931 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14931_rabbit-ewsr1-mab-s09-3a7-11320251555920.jpg)
![Western Blot: 5-Lipoxygenase Antibody (SR2214) [NBP3-21941] - Western blot analysis using NBP3-21941 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21941_rabbit-5-lipoxygenase-mab-sr2214-5720231523495.jpg "Western Blot: 5-Lipoxygenase Antibody (SR2214) [NBP3-21941] - Western blot analysis using NBP3-21941 in K562 cell lysate.")