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![Immunohistochemistry: CTLA-4 Antibody (PS01-34) [NBP3-32230] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CTLA-4 antibody (NBP3-32230) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32230) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32230_rabbit-ctla-4-mab-ps01-34-298202414161879.jpg "Immunohistochemistry: CTLA-4 Antibody (PS01-34) [NBP3-32230] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CTLA-4 antibody (NBP3-32230) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32230) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Kv4.2 Antibody [NBP2-19314] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Kv4.2-Antibody-Western-Blot-NBP2-19314-img0001.jpg "Western Blot: Kv4.2 Antibody [NBP2-19314] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: IMPDH1 Antibody [NBP1-32854] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IMPDH1-Antibody-Western-Blot-NBP1-32854-img0002.jpg "Western Blot: IMPDH1 Antibody [NBP1-32854] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry: C8B Antibody - BSA Free [NBP3-47464] - Immunohistochemistry of paraffin-embedded Rat liver tissue slide using (C8B antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-47464_rabbit-c8b-pab-bsa-free-28420251372072.jpg "Immunohistochemistry: C8B Antibody - BSA Free [NBP3-47464] - Immunohistochemistry of paraffin-embedded Rat liver tissue slide using (C8B antibody) at dilution of 1:200")
![Flow Cytometry: CD45RO Antibody (ILA116A) [NBP2-61035] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/CD45RO-Antibody-ILA116A-Flow-Cytometry-NBP2-61035-img0001.jpg "Flow Cytometry: CD45RO Antibody (ILA116A) [NBP2-61035] - Obtained with bovine leukocytes")
![Western Blot: PRAS40 [p Ser183] Antibody [NBP3-13077] - Untreated (-) and treated (+) 293T whole cell extracts (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Ser183) antibody (NBP3-13077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PRAS40-[p-Ser183]-Antibody-Western-Blot-NBP3-13077-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PRAS40 [p Ser183] Antibody [NBP3-13077] - Untreated (-) and treated (+) 293T whole cell extracts (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Ser183) antibody (NBP3-13077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Semaphorin 7A Antibody (HL1826) - Azide and BSA Free [NBP3-25677] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [HL1826] (NBP3-25677) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25677_rabbit-semaphorin-7a-mab-hl1826-azide-and-bsa-free-21202494146.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Semaphorin 7A Antibody (HL1826) - Azide and BSA Free [NBP3-25677] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [HL1826] (NBP3-25677) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: CD200R Antibody [NBP1-68990] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CD200R-Antibody-Western-Blot-NBP1-68990-img0002.jpg "Western Blot: CD200R Antibody [NBP1-68990] - Human Liver cell lysate, concentration 0.2-1 ug/ml.")