| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48609-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38683-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82484-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73221 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83162-25ul | |||
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NBP2-48609] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48609-img0018.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human cerebral cortex shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0013.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-NBP2-48609-img0019.jpg)
![Immunohistochemistry: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0012.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0014.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human testis shows no membranous positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0016.jpg)
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0004.jpg "Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunohistochemistry of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Immunohistochemistry-Paraffin-NBP3-15706-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173456.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173352.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining in human stomach and liver tissues using NBP2-38683 antibody. Corresponding SLC9A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining in human stomach and liver tissues using NBP2-38683 antibody. Corresponding SLC9A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human kidney, liver, prostate and stomach. (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38683-img0004.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human kidney shows moderate membranous/cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0011.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0013.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human prostate shows moderate membranous/cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0014.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human stomach shows strong membranous/cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP2-38683-img0015.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0018.jpg "Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0005.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining in human cerebral cortex and pancreas tissues using anti-OMG antibody. Corresponding OMG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0007.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human caudate nucleus.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0009.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human kidney.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0010.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human colon.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0011.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human caudate nucleus shows moderate to strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0013.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human cerebral cortex shows moderate positivity in neuropil, as well as staining in glial cells.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human caudate nucleus, cerebral cortex, kidney and liver using Anti-OMgp antibody NBP1-82484 (A) shows similar protein distribution across tissues to independent antibody NBP1-82483 (B).](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0016.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82484] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82484-img0017.jpg)
![Immunohistochemistry: CRYGS Antibody [NBP1-84375] - Staining of human eye shows strong cytoplasmic positivity in lens fibers cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84375_rabbit-polyclonal-crygs-antibody-immunohistochemistry-paraffin-4122024104813.png "Immunohistochemistry: CRYGS Antibody [NBP1-84375] - Staining of human eye shows strong cytoplasmic positivity in lens fibers cells.")
![Immunohistochemistry: CRYGS Antibody [NBP1-84375] - Staining of rat eye shows strong cytoplasmic positivity in lens fiber cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84375_rabbit-polyclonal-crygs-antibody-immunohistochemistry-paraffin-412202410500..png)
![Immunohistochemistry: CRYGS Antibody [NBP1-84375] - Staining of human skin shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-84375_rabbit-polyclonal-crygs-antibody-immunohistochemistry-412202410543..png)
![Immunohistochemistry: CRYGS Antibody [NBP1-84375] -Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-84375_rabbit-polyclonal-crygs-antibody-immunohistochemistry-4122024105519..png)
![Western Blot: PACSIN3 Antibody (4F8) [NBP2-03475] Analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-4F8-Western-Blot-NBP2-03475-img0005.jpg "Western Blot: PACSIN3 Antibody (4F8) [NBP2-03475] Analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.")
![Immunohistochemistry-Paraffin: PACSIN3 Antibody (4F8) [NBP2-03475] - Staining of paraffin-embedded Human prostate tissue using anti-PACSIN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-4F8-Immunohistochemistry-Paraffin-NBP2-03475-img0004.jpg)
![Flow Cytometry: PACSIN3 Antibody (4F8) [NBP2-03475] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PACSIN3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-4F8-Flow-Cytometry-NBP2-03475-img0001.jpg)
![Western Blot: PACSIN3 Antibody (4F8) [NBP2-03475] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-4F8-Western-Blot-NBP2-03475-img0002.jpg)
![Immunohistochemistry-Paraffin: PACSIN3 Antibody (4F8) [NBP2-03475] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PACSIN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-4F8-Immunohistochemistry-Paraffin-NBP2-03475-img0003.jpg)
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0003.jpg "Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Immunohistochemistry of paraffin-embedded human colon using Nrf1 Rabbit mAb (NBP3-16161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Immunohistochemistry-Paraffin-NBP3-16161-img0001.jpg)
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0002.jpg)
![Chromatin Immunoprecipitation: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Nrf1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16161_rabbit-nrf1-mab-4q4e7-52202511383725.jpg)
![Western Blot: Glut3 Antibody [NBP3-12237] - WB of NBP3-12237 with rat brain. 1:500 antibody dilution in DiluObuffer. Apparent MW is 45-47 kDa.](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12237-img0002.jpg "Western Blot: Glut3 Antibody [NBP3-12237] - WB of NBP3-12237 with rat brain. 1:500 antibody dilution in DiluObuffer. Apparent MW is 45-47 kDa.")
![Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Rat testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section.](http://images.novusbio.com/fullsize/Glut3-Antibody-Immunohistochemistry-Paraffin-NBP3-12237-img0004.jpg)
![Western Blot: Glut3 Antibody [NBP3-12237] - Cortical extracts (10 ug total protien/lane) on 10% gel. Primary antibody glucose transporter type 3 diluted 1:2000 in Dilobuffer incubation in primary anitbody 120 minutes at RT; washes 3x10 minutes in TBST secondary antibody alkaline phosphatase conjugated goat anti-rabbit IgG (1:5000)](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12237-img0001.jpg)
![Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Mouse Spleen-NBP3-12237. 1:100 dilution in IHC blocking buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section](http://images.novusbio.com/fullsize/Glut3-Antibody-Immunohistochemistry-Paraffin-NBP3-12237-img0003.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0009.jpg "Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunohistochemistry-Paraffin-H00008773-M01-img0006.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - SNAP23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0001.jpg)
![Immunoprecipitation: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 transfected lysate using anti-SNAP23 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNAP23 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunoprecipitation-H00008773-M01-img0007.jpg)
![Sandwich ELISA: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Sandwich-ELISA-H00008773-M01-img0008.jpg)
![Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-83162-img0006.jpg "Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.")
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-83162] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83162-img0008.jpg)
![Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunohistochemistry-Paraffin-NBP1-83162-img0007.jpg)