| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90022-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97412-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90536-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99490-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90470-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98717-50ul | |||
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Western Blot: Inversin Antibody [NBP3-32477] - Western blot analysis of Inversin on different lysates with Rabbit anti-Inversin antibody (NBP3-32477) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: K562 cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: 293 cell lysate (10 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 118 kDa Observed band size: 95 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32477) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32477_rabbit-inversin-pab-278202416331279.jpg "Western Blot: Inversin Antibody [NBP3-32477] - Western blot analysis of Inversin on different lysates with Rabbit anti-Inversin antibody (NBP3-32477) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: K562 cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: 293 cell lysate (10 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 118 kDa Observed band size: 95 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32477) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP2-97412] - Immunochemical staining of human Aminomethyltransferase in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Aminomethyltransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-97412-img0002.jpg "Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP2-97412] - Immunochemical staining of human Aminomethyltransferase in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP2-97412] - Immunochemical staining of human Aminomethyltransferase in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Aminomethyltransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-97412-img0001.jpg)
![Western Blot: Fc gamma RIII (CD16) Antibody (015) [NBP2-90536] - Anti-Fc gamma RIII (CD16) rabbit monoclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-015-Western-Blot-NBP2-90536-img0003.jpg "Western Blot: Fc gamma RIII (CD16) Antibody (015) [NBP2-90536] - Anti-Fc gamma RIII (CD16) rabbit monoclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Fc gamma RIII (CD16) Antibody (015) [NBP2-90536] - Fc gamma RIII (CD16) was immunoprecipitated using: Lane A: 0.5 mg RAW246.7 Whole Cell Lysate. 4ul anti-Fc gamma RIII (CD16) rabbit monoclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Fc gamma RIII (CD16) rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 50 kDax](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-015-Immunoprecipitation-NBP2-90536-img0004.jpg)
![Western Blot: beta-Catenin [p Ser33, p Ser37] Antibody (JE59-59) [NBP3-32751] - Western blot analysis of beta-Catenin on different lysates with Rabbit anti-beta-Catenin antibody (NBP3-32751) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: C6 whole cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32751) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32751_rabbit-beta-catenin-p-ser33-p-ser37-mab-je59-59-2782024163161.jpg "Western Blot: beta-Catenin [p Ser33, p Ser37] Antibody (JE59-59) [NBP3-32751] - Western blot analysis of beta-Catenin on different lysates with Rabbit anti-beta-Catenin antibody (NBP3-32751) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: C6 whole cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32751) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Kv1.5 Antibody [NBP3-30822] - Western blot analysis using Kv1.5 antibody on rat brain lysate.](http://images.novusbio.com/fullsize/nbp3-30822_rabbit-kv1-5-pab-811202420594211.jpg "Western Blot: Kv1.5 Antibody [NBP3-30822] - Western blot analysis using Kv1.5 antibody on rat brain lysate.")
![Western Blot: 14-3-3 eta Antibody [NBP2-99490] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A375 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDax](http://images.novusbio.com/fullsize/14-3-3-eta-Antibody-Western-Blot-NBP2-99490-img0003.jpg "Western Blot: 14-3-3 eta Antibody [NBP2-99490] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A375 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDax")
![Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99490] - Lane A:0.5 mg A375 Whole Cell Lysate4 uL anti-YWHAH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99490_rabbit-polyclonal-14-3-3-eta-antibody-immunoprecipitation-287202374410.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - NBP3-00350 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:31 kDa](http://images.novusbio.com/fullsize/nbp3-00350_rabbit-polyclonal-pa28-activator-beta-subunit-psme2-antibody-235202521451785.png "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - NBP3-00350 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:31 kDa")
![Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - PSME2 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-00350 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00350, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp3-00350_rabbit-polyclonal-pa28-activator-beta-subunit-psme2-antibody-235202521451773.png)
![Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse kidney with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.](http://images.novusbio.com/fullsize/CD38-Antibody-32-Immunohistochemistry-Paraffin-NBP2-90470-img0002.jpg "Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse kidney with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.")
![Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse heart with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.](http://images.novusbio.com/fullsize/CD38-Antibody-32-Immunohistochemistry-Paraffin-NBP2-90470-img0001.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 265 kDa Exposure time: 1 minutes 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33135_rabbit-acetyl-coa-carboxylase-alpha-acaca-p-ser79-mab-28820241423319.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 265 kDa Exposure time: 1 minutes 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
![Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Western-Blot-NBP2-98717-img0002.jpg "Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Numb Antibody [NBP2-98717] - Numb was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Numb rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Numb rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Immunoprecipitation-NBP2-98717-img0001.jpg)