| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37379-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12563-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11996-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26441-50ul | |||
![Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)](http://images.novusbio.com/fullsize/SLC12A3-[p-Thr53]-Antibody-Western-Blot-NBP2-60775-img0001.jpg "Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)")
![Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against HEK293 (1) and PROM1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD133-Antibody-2F8C5-Western-Blot-NBP2-52473-img0006.jpg "Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against HEK293 (1) and PROM1-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis of Hela cells using PROM1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD133-Antibody-2F8C5-Flow-Cytometry-NBP2-52473-img0001.jpg)
![Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against human PROM1 (AA: 20-108) recombinant protein. (Expected MW is 36.1 kDa)](http://images.novusbio.com/fullsize/CD133-Antibody-2F8C5-Western-Blot-NBP2-52473-img0002.jpg)
![ELISA: CD133 Antibody (2F8C5) [NBP2-52473] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/CD133-Antibody-2F8C5-ELISA-NBP2-52473-img0005.jpg)
![Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Western-Blot-NBP2-37379-img0003.jpg "Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (7D8C5) [NBP2-37379] - Immunofluorescence analysis of HepG2 cells using NRCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Immunocytochemistry-Immunofluorescence-NBP2-37379-img0004.jpg)
![Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against human NRCAM recombinant protein. (Expected MW is 32.7 kDa)](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Western-Blot-NBP2-37379-img0002.jpg)
![ELISA: NrCAM Antibody (7D8C5) [NBP2-37379] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-ELISA-NBP2-37379-img0001.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-278202416331210.jpg "Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody (JE31-53) [NBP3-32224] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRMP2 with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202410484827.jpg)
![Flow Cytometry: CRMP2 Antibody (JE31-53) [NBP3-32224] - Flow cytometric analysis of SH-SY5Y cells labeling CRMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32224, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202416372179.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-278202416285214.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-29820241435217.jpg)
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-298202414115423.jpg)
![Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-12563-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-12563-img0003.jpg)
![Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunoprecipitation-NBP3-12563-img0002.jpg)
![Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-278202416285223.jpg "Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Immunocytochemistry analysis of A549 cells labeling Galactosylceramidase/GALC with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-29202410234291.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)
![Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Western-Blot-NBP3-11996-img0001.jpg "Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of V14/2 (NBP3-11996) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11996, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11996-img0002.jpg)
![Flow Cytometry: CCR5 [p Ser337] Antibody (V14/2) - Chimeric - Azide and BSA Free [NBP3-11996] - U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11996 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11996_rabbit-monoclonal-ccr5-p-ser337-antibody-v14-2-chimeric-74202522113035.png)
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-262202414225215.jpg "Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.")
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-2722024847192.jpg)
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody (28G5) [NBP3-26441] - Staining of U251 with Pannexin-1 Antibody (28G5) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 502-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202412493632.jpg)
![Flow Cytometry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Overlay Peak curve showing A549 cells stained with Pannexin-1 Antibody (28G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202414312766.jpg)