| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03005-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38021-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03557-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61776-0.025ml | |||
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.](http://images.novusbio.com/fullsize/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-NB100-1892-img0004.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1892-img0005.jpg)
![Western Blot: KIR2.3 Antibody [NBP3-03005] - Analysis of extracts of various cell lines, using KIR2.3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/KIR2.3-Antibody-Western-Blot-NBP3-03005-img0002.jpg "Western Blot: KIR2.3 Antibody [NBP3-03005] - Analysis of extracts of various cell lines, using KIR2.3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunohistochemistry-Paraffin: KIR2.3 Antibody [NBP3-03005] - Paraffin-embedded rat brain using KIR2.3 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIR2.3-Antibody-Immunohistochemistry-Paraffin-NBP3-03005-img0001.jpg)
![Western Blot: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Analysis of extracts of various cell lines, using Pannexin-1 Rabbit mAb (NBP3-16578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-7E8T3-Western-Blot-NBP3-16578-img0002.jpg "Western Blot: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Analysis of extracts of various cell lines, using Pannexin-1 Rabbit mAb (NBP3-16578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Human esophageal cancer using Pannexin-1 Rabbit mAb (NBP3-16578) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-7E8T3-Immunohistochemistry-Paraffin-NBP3-16578-img0001.jpg)
![Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140439.jpg "Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: EAAT3 Antibody [NBP3-38021] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140454.jpg)
![Western Blot: Pannexin-1 Antibody [NBP2-82019] - Analysis of Pannexin-1 in human ovary tissue lysate with Pannexin-1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP2-82019-img0001.jpg "Western Blot: Pannexin-1 Antibody [NBP2-82019] - Analysis of Pannexin-1 in human ovary tissue lysate with Pannexin-1 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP2-82019] - Immunofluorescence of Pannexin-1 in human ovary tissue with Pannexin-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82019-img0002.jpg)
![Western Blot: Glutamate Receptor 3 Antibody [NBP3-03557] - Western blot analysis of extracts of various cell lines, using Glutamate Receptor 3 antibody (NBP3-03557) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutamate Receptor 3 Antibody-Western Blot-NBP3-03557-img0002.jpg "Western Blot: Glutamate Receptor 3 Antibody [NBP3-03557] - Western blot analysis of extracts of various cell lines, using Glutamate Receptor 3 antibody (NBP3-03557) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Glutamate Receptor 3 Antibody [NBP3-03557] - Analysis of HeLa cells using Glutamate Receptor 3 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamate-Receptor-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03557-img0001.jpg)
![Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-Western-Blot-NBP2-61776-img0003.jpg "Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-Western-Blot-NBP2-61776-img0004.jpg)
![ELISA: GluR2 Antibody (7A7D12) [NBP2-61776] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-ELISA-NBP2-61776-img0001.jpg)
![ELISA: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-ELISA-NBP2-61776-img0002.jpg)
![Western Blot: MAO-A Antibody (5D1-R) [NBP3-32566] - Western blot analysis of MAO-A on different lysates with Mouse anti-MAO-A antibody (NBP3-32566) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32566_mouse-mao-a-mab-5d1-r-278202415212434.jpg "Western Blot: MAO-A Antibody (5D1-R) [NBP3-32566] - Western blot analysis of MAO-A on different lysates with Mouse anti-MAO-A antibody (NBP3-32566) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAO-A Antibody (5D1-R) [NBP3-32566] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MAO-A antibody (NBP3-32566) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32566) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32566_mouse-mao-a-mab-5d1-r-298202413585584.jpg)
![Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-278202416331236.jpg "Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-29820241492659.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-278202415141717.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Immunocytochemistry analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1 with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202410234298.jpg)
![Flow Cytometry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Flow cytometric analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32019, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202416453266.jpg)
![Western Blot: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372] - Western blot analysis of GAT-1/SLC6A1 on different lysates with Rabbit anti-GAT-1/SLC6A1 antibody (NBP3-32372) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate (70 heat) Lane 3: Mouse brain tissue lysate Lane 4: Mouse brain tissue lysate (70 heat) Lane 5: Rat brain tissue lysate Lane 6: Rat brain tissue lysate (70 heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32372) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32372_rabbit-gat-1-slc6a1-mab-je34-61-27820241631675.jpg "Western Blot: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372] - Western blot analysis of GAT-1/SLC6A1 on different lysates with Rabbit anti-GAT-1/SLC6A1 antibody (NBP3-32372) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate (70 heat) Lane 3: Mouse brain tissue lysate Lane 4: Mouse brain tissue lysate (70 heat) Lane 5: Rat brain tissue lysate Lane 6: Rat brain tissue lysate (70 heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32372) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GAT-1/SLC6A1 antibody (NBP3-32372) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32372) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32372_rabbit-gat-1-slc6a1-mab-je34-61-298202414304137.jpg)
![Western Blot: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405] - Western blot analysis of Glutamate Receptor 6 on different lysates with Rabbit anti-Glutamate Receptor 6 antibody (NBP3-32405) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (10 ug/Lane) Lane 2: Mouse brain tissue lysate (70 heat) (10 ug/Lane) Lane 3: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32405) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32405_rabbit-glutamate-receptor-6-mab-je39-33-278202416331285.jpg "Western Blot: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405] - Western blot analysis of Glutamate Receptor 6 on different lysates with Rabbit anti-Glutamate Receptor 6 antibody (NBP3-32405) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (10 ug/Lane) Lane 2: Mouse brain tissue lysate (70 heat) (10 ug/Lane) Lane 3: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32405) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Glutamate Receptor 6 antibody (NBP3-32405) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32405) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32405_rabbit-glutamate-receptor-6-mab-je39-33-29820241420962.jpg)