| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37505-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61757-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90402-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98964-50ul | |||
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0003.jpg "Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of C6 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0004.jpg)
![Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Western-Blot-NBP3-16815-img0002.jpg "Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Immunohistochemistry-Paraffin-NBP3-16815-img0001.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-5220251242577.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human seminoma tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-8220252010493.jpg)
![Western Blot: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Western blot analysis using HEXA mouse mAb against L1210 (1), and HL7702 (2) cell lysate.](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-3F10-Western-Blot-NBP2-37505-img0003.jpg "Western Blot: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Western blot analysis using HEXA mouse mAb against L1210 (1), and HL7702 (2) cell lysate.")
![Flow Cytometry: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Flow cytometric analysis of HeLa cells using HEXA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-3F10-Flow-Cytometry-NBP2-37505-img0004.jpg)
![Western Blot: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Western blot analysis using HEXA mAb against human HEXA (AA: 29-181) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-3F10-Western-Blot-NBP2-37505-img0002.jpg)
![ELISA: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-3F10-ELISA-NBP2-37505-img0001.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0004.jpg "Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis of Hela cells using mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Flow-Cytometry-NBP2-61757-img0002.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against human Dynamin-2 (AA: 520-744) recombinant protein. (Expected MW is 28 kDa)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0003.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mouse mAb against Hela (1), U251 (2), K562 (3), Jurkat (4), and Ramos (5) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0005.jpg)
![ELISA: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-ELISA-NBP2-61757-img0001.jpg)
![Western Blot: SLC7A6 Antibody [NBP2-75086] - taining of Human Heart lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/SLC7A6-Antibody-Western-Blot-NBP2-75086-img0001.jpg "Western Blot: SLC7A6 Antibody [NBP2-75086] - taining of Human Heart lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: SLC7A6 Antibody [NBP2-75086] - Staining of paraffin embedded Human Cerebellum. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SLC7A6-Antibody-Immunohistochemistry-Paraffin-NBP2-75086-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90402-img0002.jpg "Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse kidney with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse liver with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90402-img0001.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-278202416331264.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32411) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-29820241435285.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunocytochemistry analysis of HT-29 cells labeling Glutathione S-Transferase pi 1/GSTP1 with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-2920241192326.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP3-12686-img0001.jpg "Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa")
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP3-12686] - Staining of human ATXN3 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12686-img0002.jpg)
![Immunoprecipitation: Ataxin-3 Antibody [NBP3-12686] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-ATXN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATXN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :50 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunoprecipitation-NBP3-12686-img0003.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0004.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human duodenum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0002.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human ovarian cancer with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NBP2-98964] - Immunofluorescence staining of SIN3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98964 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-98964_rabbit-polyclonal-sin3a-antibody-235202511545674.png)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-278202416285214.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-29820241435217.jpg)
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-298202414115423.jpg)