| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56234-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24575-25ul | |||
| Azide and BSA Free | NBP3-43829 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35901-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21857-50ul | |||
![Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-56234-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-56234] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56234-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: BRN3B/POU4F2 Antibody [NBP3-35901] - Immunofluorescence analysis of U2OS cells using BRN3B/POU4F2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35901_rabbit-brn3b-pou4f2-pab-1012202411592838.jpg "Immunocytochemistry/ Immunofluorescence: BRN3B/POU4F2 Antibody [NBP3-35901] - Immunofluorescence analysis of U2OS cells using BRN3B/POU4F2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: BRN3B/POU4F2 Antibody [NBP3-35901] - Western blot analysis of lysates from Mouse brain, using BRN3B/POU4F2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35901_rabbit-brn3b-pou4f2-pab-1012202412064.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: beta-Catenin [p Ser675] Antibody [NBP3-05452] - Western blot analysis of extracts of C6 cells, using beta-Catenin antibody (NBP3-05452) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser675]-Antibody-Western-Blot-NBP3-05452-img0001.jpg "Western Blot: beta-Catenin [p Ser675] Antibody [NBP3-05452] - Western blot analysis of extracts of C6 cells, using beta-Catenin antibody (NBP3-05452) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] - Immunofluorescence analysis of Rat colon(treated with lambdaPP) and Rat colon tissue using beta-Catenin Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-05452_rabbit-polyclonal-beta-catenin-p-ser675-antibody-5220251639674.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] - Immunofluorescence analysis of Mouse colon(treated with lambdaPP) and Mouse colon(untreated) tissue using beta-Catenin Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-05452_rabbit-polyclonal-beta-catenin-p-ser675-antibody-52202516283254.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19951] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: Hep G2 D: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19951-img0001.jpg "Western Blot: Proteasome 19S S5A Antibody [NBP2-19951] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: Hep G2 D: HCT116 10% SDS PAGE gel, diluted at 1:1000.")
![PSMD4 antibody [N1C2] detects PSMD4 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PSMD4 protein stained by PSMD4 antibody [N1C2] (NBP2-19951) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19951_rabbit-polyclonal-s5a-angiocidin-antibody-immunocytochemistry-immunofluorescence-261120251821101.jpg)
![Western Blot: Gemin 3 Antibody [NBP2-16638] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Western-Blot-NBP2-16638-img0001.jpg "Western Blot: Gemin 3 Antibody [NBP2-16638] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: Gemin 3 Antibody [NBP2-16638] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16638-img0002.jpg)
![Western Blot: MEK5 Antibody [NBP2-17270] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/MEK5-Antibody-Western-Blot-NBP2-17270-img0001.jpg "Western Blot: MEK5 Antibody [NBP2-17270] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: MEK5 Antibody [NBP2-17270] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MEK5-Antibody-Immunohistochemistry-Paraffin-NBP2-17270-img0002.jpg)
![Western Blot: CLN3 Antibody [NBP2-43782] - Analysis of A. 30 ug NT2D1 whole cell lysate/extract 10 % SDS-PAGE CLN3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/CLN3-Antibody-Western-Blot-NBP2-43782-img0002.jpg "Western Blot: CLN3 Antibody [NBP2-43782] - Analysis of A. 30 ug NT2D1 whole cell lysate/extract 10 % SDS-PAGE CLN3 antibody dilution: 1:1000")
![Western Blot: CLN3 Antibody [NBP2-43782] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE CLN3 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/CLN3-Antibody-Western-Blot-NBP2-43782-img0001.jpg)
![Western Blot: KSR1 Antibody (SR1095) [NBP3-21857] - Western blot analysis using NBP3-21857 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21857_rabbit-ksr1-mab-sr1095-57202316582137.jpg "Western Blot: KSR1 Antibody (SR1095) [NBP3-21857] - Western blot analysis using NBP3-21857 in HEK293 cell lysate.")