| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06071-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12617-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89383-50ul | |||
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Western-Blot-NBP2-97692-img0005.jpg "Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human skin with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0004.jpg)
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human esophagus with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0003.jpg)
![Immunoprecipitation: PCYT1A Antibody [NBP2-97692] - PCYT1A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-PCYT1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCYT1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunoprecipitation-NBP2-97692-img0006.jpg)
![Western Blot: CLCC1 Antibody [NBP3-06071] - Anti-CLCC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CLCC1-Antibody-Western-Blot-NBP3-06071-img0001.jpg "Western Blot: CLCC1 Antibody [NBP3-06071] - Anti-CLCC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: CLCC1 Antibody [NBP3-06071] - CLCC1 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-CLCC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-CLCC1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size :62 kDa](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunoprecipitation-NBP3-06071-img0002.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody - BSA Free [NBP3-06071] - Immunochemical staining of human CLCC1 in human liver with NBP3-06071 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06071_rabbit-polyclonal-clcc1-antibody-235202512205548.png)
![Immunohistochemistry-Paraffin: CLCC1 Antibody - BSA Free [NBP3-06071] - Immunochemical staining of human CLCC1 in human gallbladder with NBP3-06071 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06071_rabbit-polyclonal-clcc1-antibody-23520251129397.png)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: PIEZO1 Antibody (2-10-R-A) [NBP3-32331] - Western blot analysis of PIEZO1 on recombinant protein lysates with Mouse anti-PIEZO1 antibody (NBP3-32331) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32331) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32331_mouse-piezo1-mab-2-10-r-a-278202415242670.jpg "Western Blot: PIEZO1 Antibody (2-10-R-A) [NBP3-32331] - Western blot analysis of PIEZO1 on recombinant protein lysates with Mouse anti-PIEZO1 antibody (NBP3-32331) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32331) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PIEZO1 Antibody (2-10-R-A) [NBP3-32331] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-PIEZO1 antibody (NBP3-32331) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32331) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32331_mouse-piezo1-mab-2-10-r-a-2982024142094.jpg)
![Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10-R-A) [NBP3-32331] - Immunocytochemistry analysis of A431 cells labeling PIEZO1 with Mouse anti-PIEZO1 antibody (NBP3-32331) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-PIEZO1 antibody (NBP3-32331) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32331_mouse-piezo1-mab-2-10-r-a-29202410484894.jpg)
![Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-288202412442920.jpg "Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-298202414161898.jpg)
![Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929] - Flow cytometric analysis of SiHa cells labeling DLG1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32929, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-392024884663.jpg)
![Western Blot: Kv7.2 Antibody [NBP2-85192] - Lanes: 100 ug CHO cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit HRP. Secondary Antibody Dilution: 1:25000. Gene Name: KCNQ2. Submitted by: Anonymous.](http://images.novusbio.com/fullsize/Kv7.2-Antibody-Western-Blot-NBP2-85192-img0004.jpg "Western Blot: Kv7.2 Antibody [NBP2-85192] - Lanes: 100 ug CHO cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit HRP. Secondary Antibody Dilution: 1:25000. Gene Name: KCNQ2. Submitted by: Anonymous.")
![Immunohistochemistry: Kv7.2 Antibody [NBP2-85192] - Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5ug/ml using anti-KCNQ2 antibody](http://images.novusbio.com/fullsize/Kv7.2-Antibody-Immunohistochemistry-NBP2-85192-img0001.jpg)
![Western Blot: Kv7.2 Antibody [NBP2-85192] - Host: Mouse. Target Name: KCNQ2. Sample Tissue: Mouse Pancreas. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Kv7.2-Antibody-Western-Blot-NBP2-85192-img0003.jpg)
![Western Blot: Kv7.2 Antibody [NBP2-85192] - WB Suggested Anti-KCNQ2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Kv7.2-Antibody-Western-Blot-NBP2-85192-img0002.jpg)
![Western Blot: NDUFS5 Antibody [NBP3-12617] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:13 kDaObserved band size:13 kDa](http://images.novusbio.com/fullsize/NDUFS5-Antibody-Western-Blot-NBP3-12617-img0001.jpg "Western Blot: NDUFS5 Antibody [NBP3-12617] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:13 kDaObserved band size:13 kDa")
![Immunohistochemistry-Paraffin: NDUFS5 Antibody [NBP3-12617] - Staining of human NDUFS5 in human liver with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFS5-Antibody-Immunohistochemistry-Paraffin-NBP3-12617-img0003.jpg)
![Immunohistochemistry-Paraffin: NDUFS5 Antibody [NBP3-12617] - Staining of human NDUFS5 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFS5-Antibody-Immunohistochemistry-Paraffin-NBP3-12617-img0002.jpg)
![Immunoprecipitation: NDUFS5 Antibody [NBP3-12617] - Lane A:0.5 mg K562 Whole Cell Lysate. 4 uL anti-NDUFS5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFS5 rabbit polyclonal antibody,at 1:100 dilution .Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size :13 kDa](http://images.novusbio.com/fullsize/NDUFS5-Antibody-Immunoprecipitation-NBP3-12617-img0004.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84111] - Host: Rabbit. Target Name: KCNN2. Sample Tissue: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84111-img0001.jpg "Western Blot: KCNN2 Antibody [NBP2-84111] - Host: Rabbit. Target Name: KCNN2. Sample Tissue: Human Fetal Liver. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: KCNN2 Antibody [NBP2-84111] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN2. Gene Name: KCNN2. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-NBP2-84111-img0004.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84111] - Host: Rabbit. Target: KCNN2. Positive control (+): Human liver (LI). Negative control (-): 293T (2T). Antibody concentration: 0.5ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84111-img0002.jpg)
![Immunohistochemistry: KCNN2 Antibody [NBP2-84111] - Lanes: Rat brain section. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-biotin, streptavidin-diaminobenzidine. Secondary Antibody Dilution: 1:500. Gene Name: KCNN2. Submitted by: Dr. Amiel Rosenkranz, Rosalind Franklin University.](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-NBP2-84111-img0003.jpg)
![Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Western-Blot-NBP2-89383-img0003.jpg "Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383] - Staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunocytochemistry-Immunofluorescence-NBP2-89383-img0001.jpg)
![Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383] - Analysis of Human Galectin3 expression on HeLa cells. The cells were treated stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Flow-Cytometry-NBP2-89383-img0002.jpg)
![Immunoprecipitation: Galectin-3 Antibody (024) [NBP2-89383] - Lane A:0.5 mg mouse pancreas Whole Cell Lysate 4 uL anti-NKX3-1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-NKX3-1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.Predicted band size: 27 kDa Observed band size :30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunoprecipitation-NBP2-89383-img0004.jpg)
![Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-278202415212479.jpg "Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32714) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-298202413585529.jpg)
![Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunocytochemistry analysis of 293T cells labeling Pannexin-1 with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-292024111486.jpg)