| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35270-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99935-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90053-50ul | |||
![Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GABARAP-Antibody-5T6W5-Western-Blot-NBP3-16376-img0001.jpg "Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: GATE-16/GABARAPL2 Antibody (8Z8M8) [NBP3-16832] - Western blot analysis of extracts of various cell lines, using GATE-16/GABARAPL2 Rabbit mAb (NBP3-16832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GATE-16-GABARAPL2-Antibody-8Z8M8-Western-Blot-NBP3-16832-img0001.jpg "Western Blot: GATE-16/GABARAPL2 Antibody (8Z8M8) [NBP3-16832] - Western blot analysis of extracts of various cell lines, using GATE-16/GABARAPL2 Rabbit mAb (NBP3-16832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35270_rabbit-hcn1-pab-101220241252854.jpg "Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0003.jpg "Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0001.jpg)
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0004.jpg "Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0002.jpg)
![Immunohistochemistry: Frequenin Antibody [NBP3-10322] - Immunohistochemical analysis of human brain](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunohistochemistry-NBP3-10322-img0001.jpg "Immunohistochemistry: Frequenin Antibody [NBP3-10322] - Immunohistochemical analysis of human brain")
![Western Blot: Gemin 3 Antibody [NBP3-10425] - WB Suggested Anti-Gemin 3 Antibody Titration: 0.2-1 ug/ml. Positive Control: NIH/3T3 cell lysate](http://images.novusbio.com/fullsize/Gemin 3 Antibody-Western Blot-NBP3-10425-img0001.jpg "Western Blot: Gemin 3 Antibody [NBP3-10425] - WB Suggested Anti-Gemin 3 Antibody Titration: 0.2-1 ug/ml. Positive Control: NIH/3T3 cell lysate")
![Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/USP7-Antibody-03-Flow-Cytometry-NBP3-06427-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Western Blot: Frequenin Antibody [NBP3-10340] - Western blot analysis using NBP3-10340 on Human Thymus as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Frequenin-Antibody-Western-Blot-NBP3-10340-img0001.jpg "Western Blot: Frequenin Antibody [NBP3-10340] - Western blot analysis using NBP3-10340 on Human Thymus as a positive control. Antibody Titration: 0.2-1 ug/ml")
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Western-Blot-NBP2-99935-img0004.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa")
![Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Purine Nucleoside Phosphorylase/PNP was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg k562 Whole Cell Lysate 2 ul anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunoprecipitation-NBP2-99935-img0003.jpg)
![Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33073_mouse-uba3-mab-1b6-6-5-r-28820241419156.jpg "Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Western-Blot-NBP2-90053-img0002.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa")
![Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-PNP-3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PNP-3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 33 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Immunoprecipitation-NBP2-90053-img0001.jpg)