| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35587-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86664-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50048SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38391-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38390-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43647-25ul | |||
| Azide and BSA Free | NBP3-44160 | |||
![Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Rat liver using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-1012202411531015.jpg "Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Rat liver using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NBP3-35587] - Immunofluorescence analysis of U2OS cells using Monoamine Oxidase B(Monoamine Oxidase B(MAOB)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-1012202411552848.jpg)
![Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-101220241224180.jpg)
![Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Human liver using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-101220241243972.jpg)
![Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NBP3-35587] - Immunofluorescence analysis of L929 cells using Monoamine Oxidase B(Monoamine Oxidase B(MAOB)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-1012202411545521.jpg)
![Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NBP3-35587] - Immunofluorescence analysis of NIH/3T3 cells using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-101220241233890.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP3-35587] - Western blot analysis of lysates from HeLa cells, using Monoamine Oxidase B(MAOB) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-10122024120657.jpg)
![Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-101220241224161.jpg)
![Immunohistochemistry: Monoamine Oxidase B Antibody [NBP3-35587] - Immunohistochemistry analysis of paraffin-embedded Human colon using Monoamine Oxidase B(MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35587_rabbit-monoamine-oxidase-b-pab-10122024120690.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32426-img0011.jpg "Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0010.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0007.jpg)
![ELISA: Monoamine Oxidase B Antibody [NBP1-32426] - ELISA detection of MAOB using Monoamine Oxidase B antibody [N2C3] for capture at a concentration of 2.5 ug/mL and monoamine oxidase B for detection at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-ELISA-NBP1-32426-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-910202420134011.jpg)
![<b>Orthogonal Strategies Validation. </b>HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-141120251843448.jpg)
![HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-261120251854115.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86664-img0012.jpg "Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Western-Blot-NBP2-50048-img0002.jpg "Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of rat brain cerebellum section stained with mouse mAb to calbindin, NBP2-50048, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CLE7-homolog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84498-img0008.jpg "Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38391-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38391] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38391-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38391-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38391 (A) shows similar protein distribution across tissues to independent antibody NBP2-38390 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0009.jpg)
![Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38390-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38390] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38390-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38390-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38390 (A) shows similar protein distribution across tissues to independent antibody NBP2-38391 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0009.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024941471.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415939.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024942549.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249393563.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415961.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249371590.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249355523.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415924.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-212024935136.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249432872.jpg)
![Immunohistochemistry-Frozen: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:300. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249393591.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415945.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249342788.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249374965.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)