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![Western Blot: 14-3-3 beta/alpha Antibody (2F7O1) [NBP3-16768] - Western blot analysis of extracts of various cell lines, using 14-3-3 beta/alpha Rabbit mAb (NBP3-16768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-2F7O1-Western-Blot-NBP3-16768-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody (2F7O1) [NBP3-16768] - Western blot analysis of extracts of various cell lines, using 14-3-3 beta/alpha Rabbit mAb (NBP3-16768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: ROR beta Antibody (7J9T7) [NBP3-15895] - Western blot analysis of extracts of various cell lines, using ROR beta Rabbit mAb (NBP3-15895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/ROR-beta-Antibody-7J9T7-Western-Blot-NBP3-15895-img0001.jpg "Western Blot: ROR beta Antibody (7J9T7) [NBP3-15895] - Western blot analysis of extracts of various cell lines, using ROR beta Rabbit mAb (NBP3-15895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: Plexin A1 Antibody (2A5Z6) [NBP3-16254] - Western blot analysis of extracts of various cell lines, using Plexin A1 Rabbit mAb (NBP3-16254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-2A5Z6-Western-Blot-NBP3-16254-img0001.jpg "Western Blot: Plexin A1 Antibody (2A5Z6) [NBP3-16254] - Western blot analysis of extracts of various cell lines, using Plexin A1 Rabbit mAb (NBP3-16254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Calcium-sensing R/CaSR Overexpression Lysate [NBL1-08721] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calcium Sensing Receptor.](http://images.novusbio.com/fullsize/Calcium-Sensing-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08721-img0002.jpg "Western Blot: Calcium-sensing R/CaSR Overexpression Lysate [NBL1-08721] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calcium Sensing Receptor.")
![Western Blot: IFNAR1 [p Ser535, p Ser539] Antibody [NB300-258] - Detection of Flag immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT and 3. IFNAR1 S535A and S539A mutants. Detection with NB 300-258 is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide used as antigen but not by the dephospho form of this peptide.](http://images.novusbio.com/fullsize/IFNAR1-[p-Ser535-p-Ser539]-Antibody-Western-Blot-NB300-258-img0001.jpg "Western Blot: IFNAR1 [p Ser535, p Ser539] Antibody [NB300-258] - Detection of Flag immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT and 3. IFNAR1 S535A and S539A mutants. Detection with NB 300-258 is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide used as antigen but not by the dephospho form of this peptide.")
![Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_rabbit-mdm2-hdm2-p-ser166-mab-je64-20-288202412461655.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32747_rabbit-akt-p-ser473-mab-psh04-44-278202415242614.jpg "Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23435_rabbit-bad-p-ser112-pab-19920231410131.jpg "Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).")
![Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.](http://images.novusbio.com/fullsize/p53-Antibody-V5P4H6-B3-Immunohistochemistry-NBP2-50231-img0001.jpg "Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.")
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558) [NBP3-23348] - Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1 (lane 6). The blot was probed with mouse monoclonal Aldehyde Dehydrogenase 1-A1/ALDH1A1 M558 (lanes 1-6) at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/nbp3-23348_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-m558-199202312523632.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558) [NBP3-23348] - Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1 (lane 6). The blot was probed with mouse monoclonal Aldehyde Dehydrogenase 1-A1/ALDH1A1 M558 (lanes 1-6) at a dilution of 1:1,000.")