| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26311-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29665-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26409-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26192-50ul | |||
![Immunohistochemistry: SNAP23 Antibody (2D8) [NBP3-26311] - Image of SNAP23 Antibody (2D8) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26311_rabbit-snap23-mab-2d8-272202485638.jpg "Immunohistochemistry: SNAP23 Antibody (2D8) [NBP3-26311] - Image of SNAP23 Antibody (2D8) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-4F2-Western-Blot-NBP3-15216-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.")
![Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunoprecipitation-NBP1-78734-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.")
![Immunohistochemistry: LRRK2 Antibody (4E9) [NBP3-15156] - IHC image of LRRK2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/LRRK2-Antibody-4E9-Immunohistochemistry-NBP3-15156-img0001.jpg "Immunohistochemistry: LRRK2 Antibody (4E9) [NBP3-15156] - IHC image of LRRK2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.](http://images.novusbio.com/fullsize/PAK1-[p-Ser204]-Antibody-Western-Blot-NBP2-29665-img0001.jpg "Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser324] Antibody (4E8) [NBP3-26409] - Staining of SH-SY5Y cells with Tau [p Ser324] Antibody (4E8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26409_rabbit-tau-p-ser-324-mab-4e8-282202412493620.jpg "Immunocytochemistry/Immunofluorescence: Tau [p Ser324] Antibody (4E8) [NBP3-26409] - Staining of SH-SY5Y cells with Tau [p Ser324] Antibody (4E8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunocytochemistry/Immunofluorescence: Insulin R/CD220 Antibody (4B2) [NBP3-26192] - Staining of MCF7 Cells with Insulin R/CD220 Antibody (4B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26192_rabbit-insulin-r-cd220-mab-4b2-282202412475118.jpg "Immunocytochemistry/Immunofluorescence: Insulin R/CD220 Antibody (4B2) [NBP3-26192] - Staining of MCF7 Cells with Insulin R/CD220 Antibody (4B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")